Rapid determination of aristolochic acid I and II in Aristolochia plants   from different regions by beta-cyclodextrin-modified capillary zone   electrophoresis

doi:10.1016/j.chroma.2004.08.008

CORC > 北京大学 > 化学与分子工程学院

	Rapid determination of aristolochic acid I and II in Aristolochia plants from different regions by beta-cyclodextrin-modified capillary zone electrophoresis
	Li, W ; Gong, SX ; Wen, DW ; Che, BQ ; Liao, YP ; Liu, HW ; Feng, XF ; Hu, SL
刊名	journal of chromatography a
	2004
关键词	Chinese herbs nephropathy cyclodextrin aristolochic acid CHINESE HERBS NEPHROPATHY PERFORMANCE LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY MEDICINES FANGCHI RADIX HPLC DNA
DOI	10.1016/j.chroma.2004.08.008
英文摘要	Chinese herbs nephropathy (CHN) is a kind of severe kidney disease caused by excessively taking aristolochic acid (AA). Hence, it is essential for health security and quality control of related herbal medicines to develop an efficient method for separation and determination of these two important components in Traditional Chinese Medicines. In this study, a rapid capillary zone electrophoresis (CZE) method using 120 mM sodium borate buffer containing 10 mM beta-cyclodextrin (beta-CD) as modifier was firstly developed for the analysis of AA-I and AA-II within 4 min in some medicinal plant samples. The separation conditions including pH of running buffer, CD content in the buffer system, applied voltage and capillary temperature were systematically optimized, and two kinds of aristolochic acids in 37 herbal samples of Aristolochia plants were successfully determined with high separation efficiency, satisfactory sensitivity, repeatability and recovery. The result indicated high variability in the contents of aristolochic acids due to different species and regions. The comparison of CZE method with high performance liquid chromatography (HPLC) was also discussed. (C) 2004 Elsevier B.V. All rights reserved.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); PubMed; 41; ARTICLE; 1-2; 211-217; 1049
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199933]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, W,Gong, SX,Wen, DW,et al. Rapid determination of aristolochic acid I and II in Aristolochia plants from different regions by beta-cyclodextrin-modified capillary zone electrophoresis[J]. journal of chromatography a,2004.
APA	Li, W.,Gong, SX.,Wen, DW.,Che, BQ.,Liao, YP.,...&Hu, SL.(2004).Rapid determination of aristolochic acid I and II in Aristolochia plants from different regions by beta-cyclodextrin-modified capillary zone electrophoresis.journal of chromatography a.
MLA	Li, W,et al."Rapid determination of aristolochic acid I and II in Aristolochia plants from different regions by beta-cyclodextrin-modified capillary zone electrophoresis".journal of chromatography a (2004).