Recognition of single nucleotide polyrnorphisms using scanning potential   hairpin denaturation

doi:10.1021/ja043661v

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	Recognition of single nucleotide polyrnorphisms using scanning potential hairpin denaturation
	Wei, F ; Chen, CL ; Zhai, L ; Zhang, N ; Zhao, XS
刊名	journal of the american chemical society
	2005
关键词	MOLECULAR BEACON DNA HYBRIDIZATION SURFACES MUTATIONS PROBES
DOI	10.1021/ja043661v
英文摘要	Conventional single nucleotide polymorphism (SNP) assays, which based their detection on the stringency or temperature of the washing buffers, have encountered difficulties to distinguish a single base pair mismatch from a perfect match. In this study, scanning potential hairpin denaturation (SPHD) has been developed to detect SNP in a sensitive and reliable manner. Combined with hairpin oligonucleotide probes, scanning surface electric potential was used to induce a dissociation of double-stranded DNA around a unique "melting potential" (Vm), and it generated a high-contrast SNP recognition signal. A 21 base pair p53 gene segment was used to test this novel method. A single nucleotide mismatch to the hairpin probes caused an average of 400-800 mV difference in melting potential against the perfect match, while the error of this assay was lower than 20 mV. Experiments demonstrated that the hairpin stem was critical to the method. The concept of scanning potential hairpin denaturation could also be used extensively in different areas of nucleotide hybridization based assays.; Chemistry, Multidisciplinary; SCI(E); EI; PubMed; 0; ARTICLE; 15; 5306-5307; 127
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199790]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wei, F,Chen, CL,Zhai, L,et al. Recognition of single nucleotide polyrnorphisms using scanning potential hairpin denaturation[J]. journal of the american chemical society,2005.
APA	Wei, F,Chen, CL,Zhai, L,Zhang, N,&Zhao, XS.(2005).Recognition of single nucleotide polyrnorphisms using scanning potential hairpin denaturation.journal of the american chemical society.
MLA	Wei, F,et al."Recognition of single nucleotide polyrnorphisms using scanning potential hairpin denaturation".journal of the american chemical society (2005).