The interaction between severe acute respiratory syndrome coronavirus   3C-like proteinase and a dimeric inhibitor by capillary electrophoresis

doi:10.1016/j.ab.2005.04.027

CORC > 北京大学 > 化学与分子工程学院

	The interaction between severe acute respiratory syndrome coronavirus 3C-like proteinase and a dimeric inhibitor by capillary electrophoresis
	Ding, L ; Zhang, XX ; Wei, P ; Fan, KQ ; Lai, LH
刊名	分析生物化学
	2005
关键词	binding constants capillary electrophoresis interface inhibitor SARS 3C-like proteinase thermodynamic parameters MAIN PROTEINASE SERUM-ALBUMIN SARS BINDING DESIGN SUBSTRATE PROTEASE
DOI	10.1016/j.ab.2005.04.027
英文摘要	3C-like proteinase of severe acute respiratory syndrome (SARS) coronavirus has been demonstrated to be a key target for drug design against SARS. The interaction between SARS coronavirus 3C-like (3CL) proteinase and an octapeptide interface inhibitor was studied by affinity capillary electrophoresis (ACE). The binding constants were estimated by the change of migration time of the analytes in the buffer solution containing different concentrations of SARS 3CL proteinase. The results showed that SARS 3CL proteinase was able to complex with the octapeptide competitively, with binding constants of 2.44 x 10(4) m-(1) at 20 degrees C and 2.11 x 10(4) M-1 at 37 degrees C. In addition, the thermodynamic parameters deduced reveal that hydrophobic interaction might play major roles, along with electrostatic force, in the binding process. The ACE method used here could be developed to be an effective and simple way of applying large-scale drug screening and evaluation. (c) 2005 Elsevier Inc. All rights reserved.; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical; SCI(E); PubMed; 15; ARTICLE; 1; 159-165; 343
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199700]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Ding, L,Zhang, XX,Wei, P,et al. The interaction between severe acute respiratory syndrome coronavirus 3C-like proteinase and a dimeric inhibitor by capillary electrophoresis[J]. 分析生物化学,2005.
APA	Ding, L,Zhang, XX,Wei, P,Fan, KQ,&Lai, LH.(2005).The interaction between severe acute respiratory syndrome coronavirus 3C-like proteinase and a dimeric inhibitor by capillary electrophoresis.分析生物化学.
MLA	Ding, L,et al."The interaction between severe acute respiratory syndrome coronavirus 3C-like proteinase and a dimeric inhibitor by capillary electrophoresis".分析生物化学 (2005).