A continuous fluorescence assay for phospholipase A(2) with nontagged   lipid

doi:10.1016/j.ab.2006.01.021

CORC > 北京大学 > 化学与分子工程学院

	A continuous fluorescence assay for phospholipase A(2) with nontagged lipid
	Huang, CK ; Zhou, L ; Liu, Y ; Lai, LG
刊名	分析生物化学
	2006
关键词	human nonpancreatic secreted phospholipase A(2) fluorescence assay interfacial catalysis interfacial probe hydrophobic environment detection LINKED SUBSTRATES SIBLINKS DISPLACEMENT ASSAY FATTY-ACIDS INHIBITORS ENZYMES LEUKOTRIENE ACTIVATION RELEASE GENE
DOI	10.1016/j.ab.2006.01.021
英文摘要	Human nonpancreatic secreted phospholipase A(2) (hnps PLA(2)) is considered to be an important drug target for aritiinflammation therapy. We have established a new fluorescence assay by using 1-anilinophthalene-8-sulfonate (ANS) as an interfacial probe for hydrophobic environment detection. The fitted apparent k(cat)/K-m hnps PLA(2) is 0-0181 +/- 0,0005 RFU/mu M s. Tests oil known synthesized inhibitor gave IC50 Values similar to those from isotope-labeled assay. Because ANS is a commonly used probe for hydrophobic environment detection that needs no modification in the current assay, this strategy may be widely applicable for interfacial catalytic reactions. (c) 2006 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000236428000002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical; SCI(E); PubMed; 15; ARTICLE; 1; 11-17; 351
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199327]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Huang, CK,Zhou, L,Liu, Y,et al. A continuous fluorescence assay for phospholipase A(2) with nontagged lipid[J]. 分析生物化学,2006.
APA	Huang, CK,Zhou, L,Liu, Y,&Lai, LG.(2006).A continuous fluorescence assay for phospholipase A(2) with nontagged lipid.分析生物化学.
MLA	Huang, CK,et al."A continuous fluorescence assay for phospholipase A(2) with nontagged lipid".分析生物化学 (2006).