Analysis of phospholipid species in rat peritoneal surface layer by   liquid chromatography/electrospray ionization ion-trap mass spectrometry

doi:10.1016/j.bbalip.2006.03.022

CORC > 北京大学 > 化学与分子工程学院

	Analysis of phospholipid species in rat peritoneal surface layer by liquid chromatography/electrospray ionization ion-trap mass spectrometry
	Gao, Fei ; Tian, Xinkui ; Wen, Dawei ; Liao, Jie ; Wang, Tao ; Liu, Huwei
刊名	biochimica et biophysica acta molecular and cell biology of lipids
	2006
关键词	phospholipids peritoneal surface HPLC ion-trap mass spectrometry LIGHT-SCATTERING DETECTION ELECTROSPRAY-IONIZATION QUANTITATIVE-ANALYSIS BIOLOGICAL SAMPLES MEMBRANE PHOSPHOLIPIDS PLASMA-MEMBRANE CRUDE EXTRACTS ESI-MS/MS SEPARATION LIPIDS
DOI	10.1016/j.bbalip.2006.03.022
英文摘要	The main phospholipids in rat peritoneal Surface layer were analyzed by normal-phase high-performance liquid chromatography (HPLC) coupled with electrospray ionization (ESI) ion-trap mass spectrometry (MS). By using a silica gel column and a gradient of hexane/isopropanol/ water as mobile phase containing 5 mmol/L ammonium formate as modifiers, a baseline separation of glycerophosphoehtanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC), sphingomyelin (SM) and lyso-phosphatidylcholine (LPC) was obtained and more than 90 phospholipid constituents in rat peritoneal surface were identified and determined by on-line ion-trap MS detection. The major ethanolamine glycerophospholipids in rat peritoneal surfaces were plasmalogens that were highly enriched in polyunsaturated fatty acids at the sn-2 position. In addition, the fragmentation patterns for each phospholipid class by the ion-trap MS were discussed. (c) 2006 Elsevier B.V. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; Cell Biology; SCI(E); PubMed; 0; ARTICLE; 7; 667-676; 1761
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198867]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Gao, Fei,Tian, Xinkui,Wen, Dawei,et al. Analysis of phospholipid species in rat peritoneal surface layer by liquid chromatography/electrospray ionization ion-trap mass spectrometry[J]. biochimica et biophysica acta molecular and cell biology of lipids,2006.
APA	Gao, Fei,Tian, Xinkui,Wen, Dawei,Liao, Jie,Wang, Tao,&Liu, Huwei.(2006).Analysis of phospholipid species in rat peritoneal surface layer by liquid chromatography/electrospray ionization ion-trap mass spectrometry.biochimica et biophysica acta molecular and cell biology of lipids.
MLA	Gao, Fei,et al."Analysis of phospholipid species in rat peritoneal surface layer by liquid chromatography/electrospray ionization ion-trap mass spectrometry".biochimica et biophysica acta molecular and cell biology of lipids (2006).