Efficient purification and preconcentration of erythropoietin in human   urine by reusable immunoaffinity column

doi:10.1016/j.jchromb.2006.05.037

CORC > 北京大学 > 化学与分子工程学院

	Efficient purification and preconcentration of erythropoietin in human urine by reusable immunoaffinity column
	Mi, Jiebo ; Wang, Shan ; Ding, Xiaojie ; Guo, Zhenquan ; Zhao, Meiping ; Chang, Wenbao
刊名	journal of chromatography b analytical technologies in the biomedical and life sciences
	2006
关键词	erythropoietin (EPO) immunoaffinity column (IAC) purification preconcentration RECOMBINANT-HUMAN-ERYTHROPOIETIN MONOCLONAL-ANTIBODIES AGAROSE ANEMIA
DOI	10.1016/j.jchromb.2006.05.037
英文摘要	In this paper, an efficient method is proposed for purification and preconcentration of erythropoietin (EPO) in human urine samples. The EPO-specific immunoaffinity column (IAC) wag generated by covalent immobilization of anti-EPO polyclonal antibodies on Sepharose 4B support. The EPO-binding capacity of the IAC was found to be about 2.0 mu g (6.6 IU) per 1.5 mL of gel and the activity recoveries of EPO at low concentrations of 7.8, 10 and 120 mIU/mL by the IAC were between 78 and 86%. Substantial cleanup effect was observed when the IAC was applied to human urine samples. (c) 2006 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000241472400018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Chemistry, Analytical; SCI(E); EI; PubMed; 8; ARTICLE; 1; 125-130; 843
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198819]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Mi, Jiebo,Wang, Shan,Ding, Xiaojie,et al. Efficient purification and preconcentration of erythropoietin in human urine by reusable immunoaffinity column[J]. journal of chromatography b analytical technologies in the biomedical and life sciences,2006.
APA	Mi, Jiebo,Wang, Shan,Ding, Xiaojie,Guo, Zhenquan,Zhao, Meiping,&Chang, Wenbao.(2006).Efficient purification and preconcentration of erythropoietin in human urine by reusable immunoaffinity column.journal of chromatography b analytical technologies in the biomedical and life sciences.
MLA	Mi, Jiebo,et al."Efficient purification and preconcentration of erythropoietin in human urine by reusable immunoaffinity column".journal of chromatography b analytical technologies in the biomedical and life sciences (2006).