Electric potential induced dissociation of hybridized DNA with hairpin   motif immobilized on silicon surface

doi:10.1021/la060602h

CORC > 北京大学 > 化学与分子工程学院

	Electric potential induced dissociation of hybridized DNA with hairpin motif immobilized on silicon surface
	Wei, Fang ; Qu, Peng ; Zhai, Lin ; Chen, Chunlai ; Wang, Haifang ; Zhao, Xin Sheng
刊名	langmuir
	2006
关键词	DOUBLE-STRANDED DNA LIPOPROTEIN-LIPASE BINDING OLIGONUCLEOTIDE THERMODYNAMICS TRANSPOSITION DENATURATION RECOGNITION SENSITIVITY PICOGREEN
DOI	10.1021/la060602h
英文摘要	DNA probes were immobilized on silicon surfaces through ester condensation between the - COOH group on the surface and the terminal - OH group in the oligonucleotide, and the surface density of DNA probes against the concentration of immobilization solution was measured by radioactive labeling. The dissociation of DNA duplex on the surface by an electric potential was studied with the scanning potential hairpin denaturation/dissociation (SPHD). The influence of the stem length in the hairpin probe on the SPHD curve was systematically investigated. It was found that the capability of discrimination on single nucleotide polymorphism ( SNP) by a hairpin probe was related to the free energy of formation of the secondary structure in the probe (Delta G(ss)). In our system, when Delta G(ss) was around - 3 kcal/mol, an optimal recognition of SNP was reached and the SPHD curve was sigmoid. In contrast, the equivalent SPHD curve from a linear probe was exponential-decay alike with a poor discrimination of SNP. The concentration dependent experiments showed good linearity between the melting potential and logarithm of target concentration in the range of 1 x 10(-9) to 5 x 10(-7) M.; Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary; SCI(E); EI; PubMed; 0; ARTICLE; 14; 6280-6285; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198810]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wei, Fang,Qu, Peng,Zhai, Lin,et al. Electric potential induced dissociation of hybridized DNA with hairpin motif immobilized on silicon surface[J]. langmuir,2006.
APA	Wei, Fang,Qu, Peng,Zhai, Lin,Chen, Chunlai,Wang, Haifang,&Zhao, Xin Sheng.(2006).Electric potential induced dissociation of hybridized DNA with hairpin motif immobilized on silicon surface.langmuir.
MLA	Wei, Fang,et al."Electric potential induced dissociation of hybridized DNA with hairpin motif immobilized on silicon surface".langmuir (2006).