Rapid determination of C12-C26 non-derivatized fatty acids in human   serum by fast gas chromatography

doi:10.1002/jssc.200600344

CORC > 北京大学 > 化学与分子工程学院

	Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography
	Mengl, Zhe ; Wen, Dawei ; Sun, Dandan ; Gao, Fei ; Li, Wei ; Liao, Yiping ; Liu, Huwei
刊名	journal of separation science
	2007
关键词	fast gas chromatography free long-chain fatty acids human serum HUMAN PLASMA MASS SPECTROMETRY METHYL-ESTERS MILK-FAT FAST GC PROFILES BACTERIA SAMPLES COLUMN
DOI	10.1002/jssc.200600344
英文摘要	The concentration of long-chain fatty acids (LCFAs) in human serum is closely related to human health. It is therefore important to develop a fast, low-cost, efficient method for their determination. In this study, by using fast temperature programming and micro-bore short capillary columns, a fast gas chromatography method was developed for the direct analysis of non-derivatized LCFAs including n-dodecanoic acid to n-hexacosanic acid (C12:0-C26:0, even numbers only), linoleic acid (C18:2), oleic acid (C18:1), and erucic acid (C22:1) within 4.0 min. Method optimization including extraction and separation conditions is considered, and the analysis of real serum samples is presented. The results show that ten LCFAs were well separated with sufficient resolution, and the detection limit was in the range of 2.8-9.6 mu g/mL. The reproducibility (RSD) for both intra-day and inter-day determination was always less than 15%, and the recoveries for these LCFAs were from 63.1 to 97.0%.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000248240000017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); EI; PubMed; 11; ARTICLE; 10; 1537-1543; 30
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198259]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Mengl, Zhe,Wen, Dawei,Sun, Dandan,et al. Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography[J]. journal of separation science,2007.
APA	Mengl, Zhe.,Wen, Dawei.,Sun, Dandan.,Gao, Fei.,Li, Wei.,...&Liu, Huwei.(2007).Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography.journal of separation science.
MLA	Mengl, Zhe,et al."Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography".journal of separation science (2007).