Activity of compounds from Chinese herbal medicine Rhodiola kirilowii   (Regel) Maxim against HCVNS3 serine protease

doi:10.1016/j.antiviral.2007.06.001

CORC > 北京大学 > 化学与分子工程学院

	Activity of compounds from Chinese herbal medicine Rhodiola kirilowii (Regel) Maxim against HCVNS3 serine protease
	Zuo, Guoying ; Li, Zhengquan ; Chen, Lirong ; Xu, Xiaojie
刊名	抗病毒研究
	2007
关键词	antiviral HCVNS3-SP (-)-Epicatechin derivative Rhodiola kirilowii (Regel) maxim VIRUS TYPE-1 HIV-1 POLYPHENOLIC COMPOUNDS ANTIVIRAL COMPOUNDS VEGETABLE TANNINS PLANT-EXTRACTS GREEN TEA PROTEINASE INHIBITORS RHUBARB GLUCOSIDES
DOI	10.1016/j.antiviral.2007.06.001
英文摘要	Treatment of the chronic hepatitis C virus (HCV) infection is an unmet medical need, and the HCV NS3 serine protease (NS3-SP) has been used as an attractive target of antiviral screening against HCV. To find naturally chemical entities as lead compounds from which novel anti-HCV agents could be developed, bioassay-guided fractionation and isolation were performed on a crude ethanol extract from rhizomes of the Chinese medicinal herb Rhodiola kirilowii (Regel) Maxim using column chromatography (CC) techniques and in vitro inhibitory activity against HCV NS3-SP. The partition of the extract between water and different organic solvents led to the isolation and identification of 12 compounds in the ethyl acetate part which proved to be the most active. These compounds were tested for in vitro activity against HCV NS3-SP, among which four (-)-Epicatechin derivatives: 3,3'-Digalloylproprodelphinidin B2 (Rhodisin, 1); 3,3'-Digalloylprocyanidin 132 (2); (-)-Epigallocatechin-3-O-gallate (EGCG, 3); and (-)-Epicatechin-3-O-gallate (4, ECG) represented the most potent ones with IC50 of 0.77, 0.91, 8.51, and 18.55 mu M, respectively. Salidroside, the commonly known compounds, together with the other compounds showed no activity up to 100.0 mu M. Methylation and acylation of the hydroxyl groups of 1-4 caused a decrease of activity. Cell viability and secreted alkaline phosphatase (SEAP) activity assays with 1-4 revealed little if any toxicity. These nonpeptide inhibitors of HCV NS3-SP might serve as potential candidate anti-HCV agents. (c) 2007 Elsevier B.V. All rights reserved.; Pharmacology & Pharmacy; Virology; SCI(E); PubMed; 33; ARTICLE; 1; 86-92; 76
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198256]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zuo, Guoying,Li, Zhengquan,Chen, Lirong,et al. Activity of compounds from Chinese herbal medicine Rhodiola kirilowii (Regel) Maxim against HCVNS3 serine protease[J]. 抗病毒研究,2007.
APA	Zuo, Guoying,Li, Zhengquan,Chen, Lirong,&Xu, Xiaojie.(2007).Activity of compounds from Chinese herbal medicine Rhodiola kirilowii (Regel) Maxim against HCVNS3 serine protease.抗病毒研究.
MLA	Zuo, Guoying,et al."Activity of compounds from Chinese herbal medicine Rhodiola kirilowii (Regel) Maxim against HCVNS3 serine protease".抗病毒研究 (2007).