Detecting the inter-peptide arrangement and maturation process of   transthyretin (105-115) amyloid fibril using a FRET pair with short   Forster distance

doi:10.1016/j.bbrc.2007.08.059

CORC > 北京大学 > 化学与分子工程学院

	Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance
	Deng, Wei ; Cao, Aoneng ; Lai, Luhua
刊名	生物化学与生物物理学研究通讯
	2007
关键词	amyloid fibril formation amyloid fibril structure transthyretin (105-115) FRET parallel p-sheet electron microscope RESONANCE ENERGY-TRANSFER RECEPTOR-RELATED PROTEIN X-RAY-DIFFRACTION PRECURSOR PROTEIN INTRACELLULAR DOMAIN SINGLE-MOLECULE FLUORESCENCE AGGREGATION TRANSCRIPTION COMPLEXES
DOI	10.1016/j.bbrc.2007.08.059
英文摘要	Transthyretin (TTR) is an amyloidogenic protein involved in many mental diseases. The peptide derived from TTR (105-115) has been widely studied as a model peptide for understanding the mechanism of amyloid fibril formation. However, the detailed arrangement of this peptide in amyloid fibril is still unclear. We have studied the amyloid fibril formation process of TTR (105-115) by introducing a pair of FRET probes into the peptide with a dansyl group at the N-terminal and a tryptophan residue at the C-terminal. Our experiment demonstrated that the strands of TTR (105-115) in the same beta-sheet may be parallel and the mating sheets may be anti-parallel to each other in the amyloid fibril. The kinetics followed by FRET and EM indicated for a possible intermediate state and the distance between sheets became shorter when the intermediate amyloid fibril turns into a more matured form. (c) 2007 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000249602700025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 17; ARTICLE; 3; 689-694; 362
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198121]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Deng, Wei,Cao, Aoneng,Lai, Luhua. Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance[J]. 生物化学与生物物理学研究通讯,2007.
APA	Deng, Wei,Cao, Aoneng,&Lai, Luhua.(2007).Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance.生物化学与生物物理学研究通讯.
MLA	Deng, Wei,et al."Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance".生物化学与生物物理学研究通讯 (2007).