Antibacterial properties of recombinant human non-pancreatic secretory   phospholipase A(2)

doi:10.1016/j.bbrc.2013.10.092

CORC > 北京大学 > 化学与分子工程学院

	Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2)
	Qiu, Shunchen ; Lai, Luhua
刊名	生物化学与生物物理学研究通讯
	2013
关键词	Human non-pancreatic secretory phospholipase A(2) Bactericidal activity Gram-positive bacteria Gram-negative bacteria pH GRAM-POSITIVE BACTERIA GROUP IIA POLYMORPHONUCLEAR LEUKOCYTES INFLAMMATORY FLUID CELL-WALL DETERMINANTS INHIBITION
DOI	10.1016/j.bbrc.2013.10.092
英文摘要	Human non-pancreatic secretory phospholipase A(2) (hnpsPLA(2)) is a group IIA phospholipase A(2) which plays an important role in the innate immune response. This enzyme was found to exhibit bactericidal activity toward Gram-positive bacteria, but not Gram-negative ones. Though native hnpsPLA(2) is active over a broad pH range, it is only highly active at alkaline conditions with the optimum activity pH of about 8.5. In order to make it highly active at neutral pH, we have obtained two hnpsPLA(2) mutants, Glu89Lys and Arg100Glu that work better at neutral pH in a previous study. In the present study, we tested the bactericidal effects of the native hnpsPLA(2) and the two mutants. Both native hnpsPLA(2) and the two mutants exhibit bactericidal activity toward Gram-positive bacteria. Furthermore, they can also kill Escherichia coli, a Gram-negative bacterium. The two mutants showed better bactericidal activity for E. coli at neutral pH than the native enzyme, which is consistent with the enzyme activities. As hnpsPLA(2) is highly stable and biocompatible, it may provide a promising therapy for bacteria infection treatment or other bactericidal applications. (C) 2013 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000327290100030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 2; ARTICLE; 2; 453-456; 441
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/190054]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Qiu, Shunchen,Lai, Luhua. Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2)[J]. 生物化学与生物物理学研究通讯,2013.
APA	Qiu, Shunchen,&Lai, Luhua.(2013).Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2).生物化学与生物物理学研究通讯.
MLA	Qiu, Shunchen,et al."Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2)".生物化学与生物物理学研究通讯 (2013).