Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy | |
Ye, Qian ; Hu, Yunfei ; Jin, Changwen | |
刊名 | plos one
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2014 | |
关键词 | CLOSTRIDIUM-BEIJERINCKII FLAVODOXIN OXIDATION-REDUCTION POTENTIALS TORSION ANGLE DYNAMICS ACTIVITY IN-VITRO BACKBONE DYNAMICS ELECTRON-TRANSFER BIOTIN SYNTHASE FMN-BINDING C-13/N-15-ENRICHED PROTEINS ANABAENA APOFLAVODOXIN |
DOI | 10.1371/journal.pone.0103936 |
英文摘要 | Flavodoxins are a family of small FMN-binding proteins that commonly exist in prokaryotes. They utilize a non-covalently bound FMN molecule to act as the redox center during the electron transfer processes in various important biological pathways. Although extensive investigations were performed, detailed molecular mechanisms of cofactor binding and electron transfer remain elusive. Herein we report the solution NMR studies on Escherichia coli flavodoxins FldA and YqcA, belonging to the long-chain and short-chain flavodoxin subfamilies respectively. Our structural studies demonstrate that both proteins show the typical flavodoxin fold, with extensive conformational exchanges observed near the FMN binding pocket in their apo-forms. Cofactor binding significantly stabilizes both proteins as revealed by the extension of secondary structures in the holo-forms, and the overall rigidity shown by the backbone dynamics data. However, the 50 s loops of both proteins in the holo-form still show conformational exchanges on the mu s-ms timescales, which appears to be a common feature in the flavodoxin family, and might play an important role in structural fine-tuning during the electron transfer reactions.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000341357200047&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 4; ARTICLE; yunfei@pku.edu.cn; changwen@pku.edu.cn; 8; e103936; 9 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/162432] ![]() |
专题 | 化学与分子工程学院 生命科学学院 |
推荐引用方式 GB/T 7714 | Ye, Qian,Hu, Yunfei,Jin, Changwen. Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy[J]. plos one,2014. |
APA | Ye, Qian,Hu, Yunfei,&Jin, Changwen.(2014).Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy.plos one. |
MLA | Ye, Qian,et al."Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy".plos one (2014). |
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