The permeability and transport mechanism of graphene quantum dots (GQDs)   across the biological barrier

doi:10.1039/c4nr04136d

CORC > 北京大学 > 化学与分子工程学院

	The permeability and transport mechanism of graphene quantum dots (GQDs) across the biological barrier
	Wang, Xin-yi ; Lei, Rong ; Huang, Hong-duang ; Wang, Na ; Yuan, Lan ; Xiao, Ru-yue ; Bai, Li-dan ; Li, Xue ; Li, Li-mei ; Yang, Xiao-da
刊名	nanoscale
	2015
关键词	V)-DIPICOLINATE COMPLEXES FUNCTIONALIZED GRAPHENE CHOLESTEROL CONTENT GOLD NANOPARTICLES TARGETED DELIVERY CARBON-FIBERS CACO-2 CELLS LIPID RAFTS CAVEOLAE BRAIN
DOI	10.1039/c4nr04136d
英文摘要	As an emerging nanomaterial, graphene quantum dots (GQDs) have shown enormous potential in theranostic applications. However, many aspects of the biological properties of GQDs require further clarification. In the present work, we prepared two sizes of GQDs and for the first time investigated their membrane permeabilities, one of the key factors of all biomedical applications, and transport mechanisms on a Madin Darby Canine Kidney (MDCK) cell monolayer. The experimental results revealed that under similar to 300 mg L-1, GQDs were innoxious to MDCK and did not affect the morphology and integrity of the cell monolayer. The P-app values were determined to be 1-3 x 10(-6) cm s(-1) for the 12 nm GQDs and 0.5-1.5 x 10(-5) cm s(-1) for the 3 nm GQDs, indicating that the 3 nm GQDs are well-transported species while the 12 nm GQDs have a moderate membrane permeability. The transport and uptake of GQDs by MDCK cells were both time and concentration-dependent. Moreover, the incubation of cells with GQDs enhanced the formation of lipid rafts, while inhibition of lipid rafts with methyl-beta-cyclodextrin almost eliminated the membrane transport of GQDs. Overall, the experimental results suggested that GQDs cross the MDCK cell monolayer mainly through a lipid raft-mediated transcytosis. The present work has indicated that GQDs are a novel, low-toxic, highly-efficient general carrier for drugs and/or diagnostic agents in biomedical applications.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000348348300060&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; SCI(E); EI; PubMed; 5; ARTICLE; xyang@bjmu.edu.cn; 5; 2034-2041; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/156290]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Xin-yi,Lei, Rong,Huang, Hong-duang,et al. The permeability and transport mechanism of graphene quantum dots (GQDs) across the biological barrier[J]. nanoscale,2015.
APA	Wang, Xin-yi.,Lei, Rong.,Huang, Hong-duang.,Wang, Na.,Yuan, Lan.,...&Yang, Xiao-da.(2015).The permeability and transport mechanism of graphene quantum dots (GQDs) across the biological barrier.nanoscale.
MLA	Wang, Xin-yi,et al."The permeability and transport mechanism of graphene quantum dots (GQDs) across the biological barrier".nanoscale (2015).