Digital Polymerase Chain Reaction in an Array of Femtoliter   Polydimethylsiloxane Microreactors

doi:10.1021/ac300761n

CORC > 北京大学 > 化学与分子工程学院

	Digital Polymerase Chain Reaction in an Array of Femtoliter Polydimethylsiloxane Microreactors
	Men, Yongfan ; Fu, Yusi ; Chen, Zitian ; Sims, Peter A. ; Greenleaf, William J. ; Huang, Yanyi
刊名	analytical chemistry
	2012
关键词	INDIVIDUAL ENVIRONMENTAL BACTERIA DNA COPY NUMBER NANOFLUIDIC PLATFORM COLORECTAL CANCERS ALLELIC IMBALANCE COUNTING ALLELES EARLY-STAGE PCR MICROFLUIDICS TUMORIGENESIS
DOI	10.1021/ac300761n
英文摘要	We developed a simple, compact inicrofluidic device to perform high dynamic-range digital polymerase chain reaction (dPCR) in an array of isolated 36-femtoliter microreactors. The density of the microreactors exceeded 20 000/mm(2). This device, made from polydimethylsiloxane (PDMS), allows the samples to be loaded into all microreactors simultaneously. The microreactors are completely sealed through the deformation of a PM/ES membrane. The small volume of the microreactors ensures a compact device with high reaction efficiency and low reagent and sample consumption. Future potential applications of this platform include multicolor dPCR and massively parallel dPCR for next generation sequencing library preparation.; Chemistry, Analytical; SCI(E); EI; 0; ARTICLE; 10; 4262-4266; 84
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/154620]
专题	化学与分子工程学院工学院
推荐引用方式 GB/T 7714	Men, Yongfan,Fu, Yusi,Chen, Zitian,et al. Digital Polymerase Chain Reaction in an Array of Femtoliter Polydimethylsiloxane Microreactors[J]. analytical chemistry,2012.
APA	Men, Yongfan,Fu, Yusi,Chen, Zitian,Sims, Peter A.,Greenleaf, William J.,&Huang, Yanyi.(2012).Digital Polymerase Chain Reaction in an Array of Femtoliter Polydimethylsiloxane Microreactors.analytical chemistry.
MLA	Men, Yongfan,et al."Digital Polymerase Chain Reaction in an Array of Femtoliter Polydimethylsiloxane Microreactors".analytical chemistry (2012).