Semi-Preparative Scale Separation of Emodin from Plant Extract by Using   Molecularly Imprinted Polymer as Stationary Phase

doi:10.1007/s10337-014-2691-z

CORC > 北京大学 > 化学与分子工程学院

	Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase
	Chen, Tengfei ; Gu, Jiangyong ; Wang, Hao ; Yuan, Gu ; Chen, Lirong ; Xu, Xiaojie ; Xiao, Wei
刊名	chromatographia
	2014
关键词	Liquid chromatography Molecularly imprinted polymer Semi-preparative separation Emodin Rheum palmatum L. PERFORMANCE LIQUID-CHROMATOGRAPHY IN-SITU SYNTHESIS SAMPLE PREPARATION DRUG-DELIVERY SENSOR RECOGNITION MATRIX MICROEXTRACTION MICROSPHERES SORBENTS
DOI	10.1007/s10337-014-2691-z
英文摘要	In this work, a core-shell molecularly imprinted polymer (MIP) was synthesized through sol-gel coating procedure by using silica beads, emodin, 3-aminopropyltriethoxysilane, tetraethoxysilane and tetrahydrofuran as supporting matrix, template, functional monomer, cross-linker and solvent, respectively. The selective recognition property for emodin and its analogues (physcion and aloe emodin) of the resultant MIP was evaluated in a chromatographic column for high-performance liquid chromatography (HPLC). The retention time and imprinted factor of MIP column for emodin, physcion and aloe emodin were 5.11, 0.63, 0.69 min and 4.69, 0.75, 1.38, respectively. It showed that the MIP beads had a good binding ability for emodin. Finally, one-step separation of emodin from alcohol extract of Rheum palmatum L. at semi-preparative scale was achieved and 380 mg of emodin was collected in 5 days. The product was characterized by mass spectrometry and HPLC and its purity was 95 %.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); 2; ARTICLE; lirongc@pku.edu.cn; xiaojxu@pku.edu.cn; xw_kanion@163.com; 13-14; 893-899; 77
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/151405]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Chen, Tengfei,Gu, Jiangyong,Wang, Hao,et al. Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase[J]. chromatographia,2014.
APA	Chen, Tengfei.,Gu, Jiangyong.,Wang, Hao.,Yuan, Gu.,Chen, Lirong.,...&Xiao, Wei.(2014).Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase.chromatographia.
MLA	Chen, Tengfei,et al."Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase".chromatographia (2014).