Cupric ion enhanced molecular imprinting of bovine serum albumin in   hydrogel

doi:10.1002/jssc.200900250

CORC > 北京大学 > 化学与分子工程学院

	Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel
	Li, Sheng-Hua ; Wang, Jun ; Zhao, Mei-Ping
刊名	journal of separation science
	2009
关键词	BSA-Cu(II) complex Coordinate interaction Molecularly imprinted hydrogel ELECTROSTATIC FUNCTIONAL-GROUPS POLYACRYLAMIDE-GEL BEADS PROTEIN RECOGNITION BINDING HEMOGLOBIN HISTIDINE POLYMERS SURFACES SITE
DOI	10.1002/jssc.200900250
英文摘要	A novel molecularly imprinted hydrogel for bovine serum albumin (BSA) was prepared using cupric ion as the bridge between the template BSA and the functional monomer 4-vinylpyridine. N-Isopropylacrylamide (NIPA) was used as an assistant monomer to provide the stimuli-responsibility of the polymer. The adsorption conditions of BSA on the BSA-Cu(II)-imprinted hydrogel were optimized considering the influences of pH, temperature, and salt concentration. The proteins bound on the imprinted hydrogel can be easily recovered under mild conditions by using 10 mmol/L ethylene diamine tetraacetic acid (EDTA) (pH 7.0) containing 150 mmol/L NaCl as the eluting solution. The imprinting effect and adsorption capacity of the polymer were found to be significantly improved compared to the hydrogel prepared in the absence of cupric ion. The results demonstrated the advantages of using a template-metal ion-monomer coordination system to strengthen the interaction between the protein and monomer. The effects of different metals ions including Zn(II), Ni(II), Co(II), Cd(II), and Al(III) on the recognition ability of the BSA-Cu(II)-imprinted hydrogel were also investigated. The polymer showed high selectivity toward both the template protein and the cupric ion.; Chemistry, Analytical; SCI(E); 15; ARTICLE; 19; 3359-3363; 32
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/151312]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Sheng-Hua,Wang, Jun,Zhao, Mei-Ping. Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel[J]. journal of separation science,2009.
APA	Li, Sheng-Hua,Wang, Jun,&Zhao, Mei-Ping.(2009).Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel.journal of separation science.
MLA	Li, Sheng-Hua,et al."Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel".journal of separation science (2009).