A universal mismatch-directed signal amplification platform for   ultra-selective and sensitive DNA detection under mild isothermal   conditions

doi:10.1039/c2sc20189e

CORC > 北京大学 > 化学与分子工程学院

	A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions
	Xiao, Xianjin ; Zhang, Chen ; Su, Xin ; Song, Chen ; Zhao, Meiping
刊名	chemical science
	2012
关键词	SEQUENCE-SPECIFIC DETECTION COLI ENDONUCLEASE-IV MOLECULAR BEACONS EXONUCLEASE-III ABASIC SITES ENZYME HYBRIDIZATION LIGHTCYCLER EVOLUTION TARGETS
DOI	10.1039/c2sc20189e
英文摘要	We describe a novel mismatch-directed signal amplification method which can be performed easily under mild isothermal conditions for the ultra-selective and sensitive detection of any DNA sequence of interest. By taking advantage of the unique discrimination properties of an endonuclease IV and lambda exonuclease coupling system in cleavage reactions of DNA duplexes containing apurinic/apyrimidinic sites with different adjacent mismatched bases, the established assay enables sensitive detection of 1.0 fmol of target strand and selective differentiation of 0.5% target strand from single-base different sequences at 37 degrees C without the need for temperature adjustment.; Chemistry, Multidisciplinary; SCI(E); EI; 0; ARTICLE; 7; 2257-2261; 3
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150980]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xiao, Xianjin,Zhang, Chen,Su, Xin,et al. A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions[J]. chemical science,2012.
APA	Xiao, Xianjin,Zhang, Chen,Su, Xin,Song, Chen,&Zhao, Meiping.(2012).A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions.chemical science.
MLA	Xiao, Xianjin,et al."A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions".chemical science (2012).