Normal phase LC coupled with direct analysis in real time MS for the   chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and   jasmonic acid

doi:10.1002/elps.201200122

CORC > 北京大学 > 化学与分子工程学院

	Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid
	Chang, Cuilan ; Zhou, Zhigui ; Yang, Youyou ; Han, Yehua ; Bai, Yu ; Zhao, Meiping ; Liu, Huwei
刊名	electrophoresis
	2012
关键词	Chiral separation DART-MS Jasmonic acid 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol NPLC PERFORMANCE LIQUID-CHROMATOGRAPHY FLIGHT MASS-SPECTROMETRY ATMOSPHERIC-PRESSURE PHOTOIONIZATION DART-MS ELECTROSPRAY-IONIZATION HUMAN PLASMA ION-SOURCE S-OXIDE RESOLUTION PHARMACEUTICALS
DOI	10.1002/elps.201200122
英文摘要	Normal phase chiral LC (NPLC) has been proved to be powerful and efficient for chiral separation. However, the combination of NPLC with ESI or atmospheric pressure chemical ionization MS is restricted by the poor ionization efficiency and thermal fragmentations of analytes to some extent. Direct analysis in real time MS (DART-MS) is an ambient ionization technique that shows high ionization efficiency of the analytes in the normal phase mobile phase. In this work, we coupled chiral NPLC to DART-MS for the chiral qualitative and quantitative analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid enantiomers. Satisfactory results for the enantiomers of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol operating in the positive mode were obtained in terms of linearity (2.5250 mu g/mL, R-2, 0.999-1.000) and repeatability (25 mu g/mL, RSDs, 4.7-5.6%). Moreover, chiral NPLC-DART-MS resulted in the simultaneous chiral separation and detection of jasmonic acid enantiomers, which are very difficult to be analyzed by NPLC-ESI-MS and NPLC-APCI-MS. Compared with the coupled techniques of NPLC-ESI-MS and NPLC-APCI-MS, NPLC-DART-MS showed advantages in increasing the ionization efficiency and reducing the in-source thermal fragmentation of analytes.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); 4; ARTICLE; 22,SI; 3387-3393; 33
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150878]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Chang, Cuilan,Zhou, Zhigui,Yang, Youyou,et al. Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid[J]. electrophoresis,2012.
APA	Chang, Cuilan.,Zhou, Zhigui.,Yang, Youyou.,Han, Yehua.,Bai, Yu.,...&Liu, Huwei.(2012).Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid.electrophoresis.
MLA	Chang, Cuilan,et al."Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid".electrophoresis (2012).