Multivalent Photoaffinity Probe for Labeling Small Molecule Binding   Proteins

doi:10.1021/bc500195w

CORC > 北京大学 > 化学与分子工程学院

	Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins
	Lo, Gang ; Liu, Yu ; Yu, Xuerong ; Li, Xiaoyu
刊名	生物共轭化学
	2014
关键词	DIRECTED TOSYL CHEMISTRY COVALENT CROSS-LINKING LIGAND-TARGET PAIRS LIVING CELLS IDENTIFICATION DISCOVERY RECEPTOR COMPLEXES LIBRARIES 3-TRIFLUOROMETHYL-3-PHENYLDIAZIRINE
DOI	10.1021/bc500195w
英文摘要	Characterization of small molecule (SM)-protein interaction is of high importance in biomedical research such as target identification and proteomic profiling. Photo-cross-linking is a powerful and straightforward strategy to covalently capture SM's binding proteins. The DNA-based photoaffinity labeling method is able to capture SM's protein targets with high specificity but suffers low cross-linking efficiency, which limits its utility for low abundance and low affinity proteins. After screening a variety of cross-linkers, by utilizing the multivalency effect, the cross-linking efficiency was improved by nearly 7-fold without compromising probe specificity. The generality and performance of multivalent photoaffinity probes have been validated with a variety of SM-protein pairs in the complexity of cell lysates.; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic; SCI(E); 0; ARTICLE; xiaoyuli@pku.edu.cn; 6; 1172-1180; 25
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150468]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Lo, Gang,Liu, Yu,Yu, Xuerong,et al. Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins[J]. 生物共轭化学,2014.
APA	Lo, Gang,Liu, Yu,Yu, Xuerong,&Li, Xiaoyu.(2014).Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins.生物共轭化学.
MLA	Lo, Gang,et al."Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins".生物共轭化学 (2014).