Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis | |
Deng, Wei ; Cao, Aoneng ; Lai, Luhua | |
刊名 | 蛋白质科学
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2008 | |
关键词 | amyloid fibril FRET classification cross-beta structure elucidation |
DOI | 10.1110/ps.083475108 |
英文摘要 | The recently published microcrystal structures of amyloid fibrils from small peptides greatly enhanced our understanding of the atomic-level structure of the amyloid fibril. However, only a few amyloid fibrils can form microcrystals. The dansyl-tryptophan fluorescence resonance energy transfer (FRET) pair was shown to be able to detect the inter-peptide arrangement of the Transthyretin (105-115) amyloid fibril. In this study, we combined the known microcrystal structures with the corresponding FRET efficiencies to build a model for amyloid fibril structure classification. We found that fibrils with an antiparallel structural arrangement gave the largest FRET signal, those with a parallel arrangement gave the lowest FRET signal, and those with a mixed arrangement gave a moderate FRET signal. This confirms that the amyloid fibril structure patterns can be classified based on the FRET efficiency.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000256166600015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); PubMed; 9; ARTICLE; 6; 1102-1105; 17 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/150234] ![]() |
专题 | 化学与分子工程学院 |
推荐引用方式 GB/T 7714 | Deng, Wei,Cao, Aoneng,Lai, Luhua. Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis[J]. 蛋白质科学,2008. |
APA | Deng, Wei,Cao, Aoneng,&Lai, Luhua.(2008).Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis.蛋白质科学. |
MLA | Deng, Wei,et al."Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis".蛋白质科学 (2008). |
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