Distinguishing the cross-beta spine arrangements in amyloid fibrils   using FRET analysis

doi:10.1110/ps.083475108

CORC > 北京大学 > 化学与分子工程学院

	Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis
	Deng, Wei ; Cao, Aoneng ; Lai, Luhua
刊名	蛋白质科学
	2008
关键词	amyloid fibril FRET classification cross-beta structure elucidation
DOI	10.1110/ps.083475108
英文摘要	The recently published microcrystal structures of amyloid fibrils from small peptides greatly enhanced our understanding of the atomic-level structure of the amyloid fibril. However, only a few amyloid fibrils can form microcrystals. The dansyl-tryptophan fluorescence resonance energy transfer (FRET) pair was shown to be able to detect the inter-peptide arrangement of the Transthyretin (105-115) amyloid fibril. In this study, we combined the known microcrystal structures with the corresponding FRET efficiencies to build a model for amyloid fibril structure classification. We found that fibrils with an antiparallel structural arrangement gave the largest FRET signal, those with a parallel arrangement gave the lowest FRET signal, and those with a mixed arrangement gave a moderate FRET signal. This confirms that the amyloid fibril structure patterns can be classified based on the FRET efficiency.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000256166600015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); PubMed; 9; ARTICLE; 6; 1102-1105; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150234]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Deng, Wei,Cao, Aoneng,Lai, Luhua. Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis[J]. 蛋白质科学,2008.
APA	Deng, Wei,Cao, Aoneng,&Lai, Luhua.(2008).Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis.蛋白质科学.
MLA	Deng, Wei,et al."Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis".蛋白质科学 (2008).