An acid-labile block copolymer of PDMAEMA and PEG as potential carrier   for intelligent gene delivery systems

doi:10.1021/bm7008747

CORC > 北京大学 > 化学与分子工程学院

	An acid-labile block copolymer of PDMAEMA and PEG as potential carrier for intelligent gene delivery systems
	Lin, Song ; Du, Fusheng ; Wang, Yang ; Ji, Shouping ; Liang, Dehai ; Yu, Lei ; Li, Zichen
刊名	biomacromolecules
	2008
关键词	DNA COMPLEXES IN-VITRO POLYETHYLENIMINE/DNA COMPLEXES STERIC STABILIZATION NONVIRAL VECTORS HIGHLY EFFICIENT THERAPY POLYMER PH POLYPLEXES
DOI	10.1021/bm7008747
英文摘要	Intelligent gene delivery systems based on physiologically triggered reversible shielding technology have evinced enormous interest due to their potential in vivo applications. In the present work, an acid-labile block copolymer consisting of poly(ethylene glycol) and poly(2-(dimethylamino)ethyl methacrylate) segments connected through a cyclic ortho ester linkage (PEG-a-PDMAEMA) was synthesized by atom transfer radical polymerization of DMAEMA using a PEG macroinitiator with an acid-cleavable end group. PEG-a-PDMAEMA condensed with plasmid DNA formed polyplex nanoparticles with an acid-triggered reversible PEG shield. The pH-dependent shielding/deshielding effect of PEG chains on the polyplex particles were evaluated by potential and size measurements. At pH 7.4, polyplexes generated from PEG-a-PDMAEMA exhibited smaller particle size, lower surface charge, reduced interaction with erythrocytes, and less cytotoxicity compared to PDMAEMA-derived polyplexes. At pH 5.0, zeta potential of polyplexes formed from PEG-a-PDMAEMA increased, leveled up after 2 h of incubation and gradual aggregation occurred in the presence of bovine serum albumin (BSA). In contrast, the stably shielded polyplexes formed by DNA and an acid-stable block copolymer, PEG-b-PDMAEMA, did not change in size and zeta potential in 6 h. In vitro transfection efficiency of the acid-labile copolymer greatly increased after 6 h incubation at pH 5.0, approaching the same level of PDMAEMA, whereas there was only slight increase in efficiency for the stable copolymer, PEG-b-PDMAEMA.; Biochemistry & Molecular Biology; Chemistry, Organic; Polymer Science; SCI(E); EI; PubMed; 126; ARTICLE; 1; 109-115; 9
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150196]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Lin, Song,Du, Fusheng,Wang, Yang,et al. An acid-labile block copolymer of PDMAEMA and PEG as potential carrier for intelligent gene delivery systems[J]. biomacromolecules,2008.
APA	Lin, Song.,Du, Fusheng.,Wang, Yang.,Ji, Shouping.,Liang, Dehai.,...&Li, Zichen.(2008).An acid-labile block copolymer of PDMAEMA and PEG as potential carrier for intelligent gene delivery systems.biomacromolecules.
MLA	Lin, Song,et al."An acid-labile block copolymer of PDMAEMA and PEG as potential carrier for intelligent gene delivery systems".biomacromolecules (2008).