Spectroscopy Probing of the Formation, Recognition, and Conversion of a   G-Quadruplex in the Promoter Region of the bcl-2 Oncogene

doi:10.1002/chem.200801922

CORC > 北京大学 > 化学与分子工程学院

	Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene
	Li, Huihui ; Liu, Yiquan ; Lin, Sen ; Yuan, Gu
刊名	chemistry a european journal
	2009
关键词	DNA structures duplexes G-quadruplexes noncovalent interactions oncogenes IONIZATION MASS-SPECTROMETRY AUTOMATED DOCKING SMALL-MOLECULE DNA BINDING PROTEINS DUPLEX DISTAMYCIN TRANSITION EXPRESSION
DOI	10.1002/chem.200801922
英文摘要	This study has demonstrated the formation of the G-quadruplex structure from the G-rich sequence in the promoter region of the bcl-2 oncogene; the formation could be induced by addition of NH(4)(+) or K(+) ions. The binding affinity and stoichiometry of seven Small Molecules With the G-quadruplex were examined by using F,SI-MS. its well as CD and UV spectroscopy. The binding-affinity order was determined to be P1 approximate to P5 > P2 > P3 approximate to P4 > P7 > P6. In particular, the small-molecule induction of the structural transition between the G-quadruplex and duplex DNA forms in this promoter region was investigated by ESI-MS. We directly observed specific binding of dehydrocorydaline (P7) and cationic porphyrin (P5) in one system consisting of the G-quadruplex and the duplex DNA, respectively. The results indicate that P7 selectively stabilizes the G-quadruplex and shifts the equalibrium toward G-quadruplex formation of the bcl-2 promoter, whereas P5 converts the G-quadruplex into the duplex DNA, which results in strong and selective binding to the duplex form. Therefore, PS and P7 with their attractive binding specificities could be considered as precursors for pathway-specific drug, design for regulation of bcl-2 oncogene transcription.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000264029500026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Multidisciplinary; SCI(E); EI; PubMed; 28; ARTICLE; 10; 2445-2452; 15
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150136]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Huihui,Liu, Yiquan,Lin, Sen,et al. Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene[J]. chemistry a european journal,2009.
APA	Li, Huihui,Liu, Yiquan,Lin, Sen,&Yuan, Gu.(2009).Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene.chemistry a european journal.
MLA	Li, Huihui,et al."Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene".chemistry a european journal (2009).