LEC-BiFC: a new method for rapid assay of protein interaction

doi:10.3109/10520295.2010.483068

CORC > 北京大学 > 化学与分子工程学院

	LEC-BiFC: a new method for rapid assay of protein interaction
	Lin, J. ; Wang, N. ; Li, Y. ; Liu, Z. ; Tian, S. ; Zhao, L. ; Zheng, Y. ; Liu, S. ; Li, S. ; Jin, C. ; Xia, B.
刊名	biotechnic histochemistry
	2011
关键词	Bak Bcl-X(L) BiFC bimolecular fluorescence complementation LEC LEC-BiFC linear expression cassette protein interaction BIMOLECULAR FLUORESCENCE COMPLEMENTATION IN-VIVO LIVING CELLS FRAGMENT VISUALIZATION POLYMERASE
DOI	10.3109/10520295.2010.483068
英文摘要	Protein-protein interactions play fundamental roles in most biological processes. Bimolecular fluorescence complementation (BiFC) is a promising method for its simplicity and direct visualization of protein-protein interactions in cells. This method, however, is limited by background fluorescence that appears without specific interaction between the proteins. We report here a point mutation (V150L) in one Venus BiFC fragment that efficiently decreases background fluorescence of BiFC assay. Furthermore, by combining this modified BiFC and linear expression cassette (LEC), we develop a simple and rapid method (LEC-BiFC) for protein interaction analysis that is demonstrated by a case study of the interaction between Bcl-X(L) and Bak BH3 peptide. The total analysis procedure can be completed in two days for screening tens of mutants. LEC-BiFC can be applied easily in any lab equipped with a fluorescence microscope.; Biotechnology & Applied Microbiology; Cell Biology; SCI(E); PubMed; 7; ARTICLE; 4; 272-279; 86
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150069]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Lin, J.,Wang, N.,Li, Y.,et al. LEC-BiFC: a new method for rapid assay of protein interaction[J]. biotechnic histochemistry,2011.
APA	Lin, J..,Wang, N..,Li, Y..,Liu, Z..,Tian, S..,...&Xia, B..(2011).LEC-BiFC: a new method for rapid assay of protein interaction.biotechnic histochemistry.
MLA	Lin, J.,et al."LEC-BiFC: a new method for rapid assay of protein interaction".biotechnic histochemistry (2011).