Improvement of reproducibility and sensitivity of CE analysis by using   the capillary coated dynamically with carboxymethyl chitosan

doi:10.1007/s00216-011-4659-2

CORC > 北京大学 > 化学与分子工程学院

	Improvement of reproducibility and sensitivity of CE analysis by using the capillary coated dynamically with carboxymethyl chitosan
	Liu, Yi ; Fu, Xiaofang ; Bai, Yu ; Zhai, Maolin ; Liao, Yiping ; Liao, Jie ; Liu, Huwei
刊名	分析化学和生物分析化学
	2011
关键词	CE-MS Carboxymethyl chitosan Dynamic coating Reproducibility Sensitivity ELECTROPHORESIS-MASS SPECTROMETRY ARISTOLOCHIC ACID-I ZONE-ELECTROPHORESIS PROTEIN SEPARATIONS INORGANIC ANIONS LIQUID-CHROMATOGRAPHY ELECTROKINETIC CHROMATOGRAPHY ELECTROOSMOTIC FLOW SURFACTANT COATINGS BASIC-PROTEINS
DOI	10.1007/s00216-011-4659-2
英文摘要	Analysis reproducibility and detection sensitivity of capillary electrophoresis (CE) are often questioned by applied scientists, which has hindered its application as a routine method. To address these issues, a simple, precise, and reproducible dynamic coating method was developed by applying carboxymethyl chitosan (CMC) dynamic coating on fused silica capillary. The proposed coating was accomplished by simply rinsing the capillary with CMC solution for 1 min in between runs, with no regeneration procedure or buffer additives needed. Electroosmotic flow could be well controlled by adjusting the pH of background electrolyte, and the adsorption of analytes onto the capillary inner wall was effectively eliminated. The main parameters of the coating condition were optimized, and extensive applications of these CMC-dynamically coated capillaries in CE separations were then firmly confirmed. By using proteins, aristolochic acids, and inorganic anions as model analytes, the coating showed a good stability, high reproducibility, as well as improved sensitivity. Baseline separations could be obtained with high efficiency. The reduced adsorption was impressively effective for basic proteins, with an average plate number of 90,000/m for each protein, apart from the good resolution on the chromatogram. A high sensitive detection of alpha-lactalbumin was achieved with a limit of detection (S/N = 3) of 3.5 nM, and the number of theoretical plates was as high as 1,200,000/m. In addition, the combination of the CMC coating with nonaqueous CE and CE-mass spectrometry proved to be practical. All results showed that the CMC-dynamically coated capillary has special properties and obvious superiority over the uncoated ones for CE analysis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000287576700022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Chemistry, Analytical; SCI(E); EI; PubMed; 15; ARTICLE; 8; 2821-2829; 399
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/150011]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Yi,Fu, Xiaofang,Bai, Yu,et al. Improvement of reproducibility and sensitivity of CE analysis by using the capillary coated dynamically with carboxymethyl chitosan[J]. 分析化学和生物分析化学,2011.
APA	Liu, Yi.,Fu, Xiaofang.,Bai, Yu.,Zhai, Maolin.,Liao, Yiping.,...&Liu, Huwei.(2011).Improvement of reproducibility and sensitivity of CE analysis by using the capillary coated dynamically with carboxymethyl chitosan.分析化学和生物分析化学.
MLA	Liu, Yi,et al."Improvement of reproducibility and sensitivity of CE analysis by using the capillary coated dynamically with carboxymethyl chitosan".分析化学和生物分析化学 (2011).