The gold-nanoparticle-based surface plasmon resonance light scattering   and visual DNA aptasensor for lysozyme

doi:10.1007/s00216-011-4943-1

CORC > 北京大学 > 化学与分子工程学院

	The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme
	Wang, Xinyi ; Xu, Yao ; Chen, Yang ; Li, Limei ; Liu, Feng ; Li, Na
刊名	分析化学和生物分析化学
	2011
关键词	Gold nanoparticles Aggregation Lysozyme DNA aptamer Plasmon resonance light scattering Visual detection COLORIMETRIC DETECTION LABEL-FREE APTAMER IMMUNOASSAY PROTEIN PROBE ASSAY POLYNUCLEOTIDES SEQUENCES SELECTION
DOI	10.1007/s00216-011-4943-1
英文摘要	We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS) measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced plasmon resonance light scattering. The linear range was found to be 0.2 similar to 4 nM for 0.7 nM AuNPs, 0.3 similar to 6 nM for 1.4 nM AuNPs and 0.6 similar to 8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection, 1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); PubMed; 23; ARTICLE; 7; 2085-2091; 400
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149982]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Xinyi,Xu, Yao,Chen, Yang,et al. The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme[J]. 分析化学和生物分析化学,2011.
APA	Wang, Xinyi,Xu, Yao,Chen, Yang,Li, Limei,Liu, Feng,&Li, Na.(2011).The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme.分析化学和生物分析化学.
MLA	Wang, Xinyi,et al."The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme".分析化学和生物分析化学 (2011).