Evaluation of the adjuvant effect of silver nanoparticles both in vitro   and in vivo

doi:10.1016/j.toxlet.2013.02.010

CORC > 北京大学 > 化学与分子工程学院

	Evaluation of the adjuvant effect of silver nanoparticles both in vitro and in vivo
	Xu, Yingying ; Tang, Huan ; Liu, Jia-hui ; Wang, Haifang ; Liu, Yuanfang
刊名	毒理学快报
	2013
关键词	Silver nanoparticles Adjuvant effect Ovalbumin Antibody Peritoneal macrophages VACCINE-DELIVERY TOXICITY ORGANISMS MICE DNA
DOI	10.1016/j.toxlet.2013.02.010
英文摘要	The immunological adjuvant effect of silver nanoparticles (AgNPs) was investigated both in vitro and in vivo. The in vivo adjuvant effect of AgNPs was evaluated with model antigen ovalbumin (OVA) and bovine serum albumin (BSA) in mice by intraperitoneal and subcutaneous immunization. Serum antigen-specific IgG level significantly increased in AgNPs-treated mice comparing to the control group. AgNPs induced the increase of IgG1/IgG2a ratio and antigen-specific IgE, indicating that AgNPs elicited Th2-biased immune responses. By in vitro assay, the mechanism of adjuvant effect was explored. After 48 h treatment with AgNPs, both the number of leukocytes and levels of cytokines TNF-alpha and IFN-gamma in abdominal lavage fluid of mice increased. The expression of the major histocompatibility complex class II molecule on the surface of peritoneal macrophages significantly increased. AgNPs can be easily phagocytosed by peritoneal macrophages, while do not affect antigen uptake by the cell. We therefore conclude that AgNPs have significant adjuvant effect and the mechanism of this effect is mainly ascribed to the recruitment and activation of local leukocytes and especially macrophages. For the first time we found the remarkable adjuvant effect of AgNPs, and the result is beneficial for the future applications, especially in biomedicine. (C) 2013 Elsevier Ireland Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000317348100006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Toxicology; SCI(E); PubMed; 23; ARTICLE; 1; 42-48; 219
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149816]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xu, Yingying,Tang, Huan,Liu, Jia-hui,et al. Evaluation of the adjuvant effect of silver nanoparticles both in vitro and in vivo[J]. 毒理学快报,2013.
APA	Xu, Yingying,Tang, Huan,Liu, Jia-hui,Wang, Haifang,&Liu, Yuanfang.(2013).Evaluation of the adjuvant effect of silver nanoparticles both in vitro and in vivo.毒理学快报.
MLA	Xu, Yingying,et al."Evaluation of the adjuvant effect of silver nanoparticles both in vitro and in vivo".毒理学快报 (2013).