Lipid profiling of human plasma from peritoneal dialysis patients using   an improved 2D (NP/RP) LC-QToF MS method

doi:10.1007/s00216-013-7109-5

CORC > 北京大学 > 化学与分子工程学院

	Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method
	Li, Min ; Feng, Baosheng ; Liang, Yuan ; Zhang, Wei ; Bai, Yu ; Tang, Wen ; Wang, Tao ; Liu, Huwei
刊名	分析化学和生物分析化学
	2013
关键词	Lipidomics Free fatty acids Phospholipids Two-dimensional liquid chromatography-mass spectrometry (2D LC-MS) Potential biomarkers MIA syndrome CHRONIC-RENAL-FAILURE MASS-SPECTROMETRY STRONG ASSOCIATION LIQUID FLUID
DOI	10.1007/s00216-013-7109-5
英文摘要	An improved online two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (2D LC-QToF MS) system was developed for the lipid profiling of human plasma, in which different lipid classes were separated by the first dimensional normal-phase (NP) LC and different lipid molecular species were separated by the second dimensional reversed-phase (RP) LC. This 2D LC-QToF MS system was built based on a ten-port, two-position valve as the interface, the conditions of which had been optimized and discussed in detail. As two loops were used to trap and transfer the first dimensional elute to the second dimension separately, this new interface suppressed the sample band broadening in the first dimensional column, increased the recovery and repeatability of 2D LC interface, and offered the possibility for the realization of not-stop-flow NP/RP 2D LC system. Finally, 190 endogenous lipid species out of 10 lipid classes were determined within a single run from the plasma of peritoneal patients. This method was also applied to identify the difference in lipid profile between plasma from peritoneal dialysis patients with bad volume status and peritoneal dialysis patients with good volume status. The discovery of 30 potential biomarkers would be helpful to the malnutrition, inflammation, and atherosclerosis syndrome investigation.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); EI; PubMed; 7; ARTICLE; 21; 6629-6638; 405
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149791]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Min,Feng, Baosheng,Liang, Yuan,et al. Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method[J]. 分析化学和生物分析化学,2013.
APA	Li, Min.,Feng, Baosheng.,Liang, Yuan.,Zhang, Wei.,Bai, Yu.,...&Liu, Huwei.(2013).Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method.分析化学和生物分析化学.
MLA	Li, Min,et al."Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method".分析化学和生物分析化学 (2013).