Combination of a modified block PCR and endonuclease IV-based signal   amplification system for ultra-sensitive detection of low-abundance   point mutations

doi:10.1016/j.ymeth.2013.06.031

CORC > 北京大学 > 化学与分子工程学院

	Combination of a modified block PCR and endonuclease IV-based signal amplification system for ultra-sensitive detection of low-abundance point mutations
	Xiao, Xianjin ; Xu, Anqin ; Zhai, Junqiu ; Zhao, Meiping
刊名	methods
	2013
关键词	Modified block PCR Endo IV-based signal amplification system Point mutation detection Abasic site-containing DNA fluorescent probe REAL-TIME PCR KRAS MUTATION COLORECTAL-CANCER CHAIN-REACTION DNA RESOLUTION PROBES GENES SELECTIVITY ENRICHMENT
DOI	10.1016/j.ymeth.2013.06.031
英文摘要	By combination of a modified block PCR and endonuclease IV-based signal amplification system, we have developed a novel approach for ultra-sensitive detection of point mutations. The method can effectively identify mutant target sequence immersed in a large background of wild-type sequences with abundance down to 0.03% (for C -> A) and 0.005% (for C -> G). This sensitivity is among the highest in comparison with other existing approaches and the operating procedures are simple and time saving. The method holds great potential for future application in clinical diagnosis and biomedical research. (c) 2013 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000328309600011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; SCI(E); PubMed; 2; ARTICLE; 3; 255-259; 64
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149787]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xiao, Xianjin,Xu, Anqin,Zhai, Junqiu,et al. Combination of a modified block PCR and endonuclease IV-based signal amplification system for ultra-sensitive detection of low-abundance point mutations[J]. methods,2013.
APA	Xiao, Xianjin,Xu, Anqin,Zhai, Junqiu,&Zhao, Meiping.(2013).Combination of a modified block PCR and endonuclease IV-based signal amplification system for ultra-sensitive detection of low-abundance point mutations.methods.
MLA	Xiao, Xianjin,et al."Combination of a modified block PCR and endonuclease IV-based signal amplification system for ultra-sensitive detection of low-abundance point mutations".methods (2013).