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	Recent advances in magic angle spinning solid state NMR of membrane proteins
	Wang, Shenlin ; Ladizhansky, Vladimir
	2014
关键词	Magic angle spinning solid-state NMR Membrane protein Protein structure Lipid bilayer Multidimensional NMR NUCLEAR-MAGNETIC-RESONANCE INFLUENZA-A VIRUS PARAMAGNETIC RELAXATION ENHANCEMENT ANABAENA SENSORY RHODOPSIN M2 PROTON CHANNEL RESOLUTION STRUCTURE DETERMINATION 7-HELICAL TRANSMEMBRANE PROTEIN SHIFT CORRELATION SPECTROSCOPY RANGE STRUCTURAL RESTRAINTS CHIMERIC POTASSIUM CHANNEL
DOI	10.1016/j.pnmrs.2014.07.001
英文摘要	Membrane proteins mediate many critical functions in cells. Determining their three-dimensional structures in the native lipid environment has been one of the main objectives in structural biology. There are two major NMR methodologies that allow this objective to be accomplished. Oriented sample NMR, which can be applied to membrane proteins that are uniformly aligned in the magnetic field, has been successful in determining the backbone structures of a handful of membrane proteins. Owing to methodological and technological developments, Magic Angle Spinning (MAS) solid-state NMR (ssNMR) spectroscopy has emerged as another major technique for the complete characterization of the structure and dynamics of membrane proteins. First developed on peptides and small microcrystalline proteins, MAS ssNMR has recently been successfully applied to large membrane proteins. In this review we describe recent progress in MAS ssNMR methodologies, which are now available for studies of membrane protein structure determination, and outline a few examples, which highlight the broad capability of ssNMR spectroscopy. (C) 2014 Elsevier B.V. All rights reserved.; Chemistry, Physical; Physics, Atomic, Molecular & Chemical; Spectroscopy; SCI(E); EI; PubMed; 0; REVIEW; wangshenlin@pku.edu.cn; vladizha@uoguelph.ca; 1-26; 82
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/149647]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Shenlin,Ladizhansky, Vladimir. Recent advances in magic angle spinning solid state NMR of membrane proteins[J],2014.
APA	Wang, Shenlin,&Ladizhansky, Vladimir.(2014).Recent advances in magic angle spinning solid state NMR of membrane proteins..
MLA	Wang, Shenlin,et al."Recent advances in magic angle spinning solid state NMR of membrane proteins".(2014).

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