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	罂粟花粉中水溶性肽的分离纯化、序列测定和合成的研究; STUDIES ON THE SEPARATION, SEQUENCE DETERMINATION AND SYNTHESIS OF THE WATER-SOLUBLE PEPTIDES FROM PAPAVER-SOMNIFERUM L
	许家喜 ; 金声
	1995
关键词	罂粟花粉 多肽 序列分析 固相合成
英文摘要	从罂粟花粉中分离得到含21,17,13和16个氨基酸残基的四种肽PSPP1～4,测定了它们的序列.用固相法合成了PSPP2～4,经HPLC和电泳法对比,确定了分离肽的一级结构.用FT-IR、CD谱初步讨论了它们在固态和溶液中的二级结构.通过T-淋巴细胞转化实验等生理活性测定方法表明它们具有一定的促免疫活性.; Four peptides PSPP 1 similar to 4, with 21, 17, 13 and 16 amino acid residues in the proper order, were obtained from the bee-collected pollen of Papaver Somniferum after freezed pollen wall-destroyed extraction, desugarization on the strong acid sulfonated ion exchange resin, gel filtration on Sephadex G25 and separation on the cationexchange resin, Their sequences have been determined by Edman degration-N-terminal dansylation. PSPP 2 similar to 4 were synthesized using solid phase method and their secondary structures in solid phase and in solutions were studied by FT-IR and CD spectra. The E-rosette and T-lymphocyte transformation tests in vitro showed that they had strong immunopromotive activies.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1995TA12700017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 4; 08; 822-827; 53
语种	中文
出处	SCI ; 知网
出版者	化学学报
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/81160]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	许家喜,金声. 罂粟花粉中水溶性肽的分离纯化、序列测定和合成的研究, STUDIES ON THE SEPARATION, SEQUENCE DETERMINATION AND SYNTHESIS OF THE WATER-SOLUBLE PEPTIDES FROM PAPAVER-SOMNIFERUM L. 1995-01-01.

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