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	Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency
	Yang, Yang ; Liu, Bei ; Xu, Jun ; Wang, Jinlin ; Wu, Jun ; Shi, Cheng ; Xu, Yaxing ; Dong, Jiebin ; Wang, Chengyan ; Lai, Weifeng ; Zhu, Jialiang ; Xiong, Liang ; Zhu, Dicong ; Li, Xiang ; Yang, Weifeng ; Yamauchi, Takayoshi ; Sugawara, Atsushi ; Li, Zhongwei ; Sun, Fangyuan ; Li, Xiangyun ; Li, Chen ; He, Aibin ; Du, Yaqin ; Wang, Ting ; Zhao, Chaoran ; Li, Haibo ; Chi, Xiaochun ; Zhang, Hongquan ; Liu, Yifang ; Li, Cheng ; Duo, Shuguang ; Yin, Ming ; Shen, Huan ; Belmonte, Juan Carlos Izpisua ; Deng, Hongkui
	2017
关键词	LARGE GENE LISTS MOUSE EMBRYOS GROUND-STATE ES CELLS INTERSPECIES CHIMERAS CULTURE-CONDITIONS RAT BLASTOCYSTS SEQUENCING DATA RNA-SEQ NAIVE
英文摘要	Of all known cultured stem cell types, pluripotent stem cells (PSCs) sit atop the landscape of developmental potency and are characterized by their ability to generate all cell types of an adult organism. However, PSCs show limited contribution to the extraembryonic placental tissues in vivo. Here, we show that a chemical cocktail enables the derivation of stem cells with unique functional and molecular features from mice and humans, designated as extended pluripotent stem (EPS) cells, which are capable of chimerizing both embryonic and extraembryonic tissues. Notably, a single mouse EPS cell shows widespread chimeric contribution to both embryonic and extraembryonic lineages in vivo and permits generating single-EPS-cell-derived mice by tetraploid complementation. Furthermore, human EPS cells exhibit interspecies chimeric competency in mouse conceptuses. Our findings constitute a first step toward capturing pluripotent stem cells with extraembryonic developmental potentials in culture and open new avenues for basic and translational research.; National Key Research and Development Program of China [2016YFA0100100]; National Natural Science Foundation of China [31521004]; Guangdong Innovative and Entrepreneurial Research Team Program [2014ZT05S216]; Science and Technology Planning Project of Guangdong Province, China [2014B020226001]; Science and Technology Program of Guangzhou, China [2016B030232001]; Ministry of Education of China (111 Project); BeiHao Stem Cell and Regenerative Medicine Translational Research Institute; Joint Institute of Peking University Health Science Center and University of Michigan Health System; National Science and Technology Support Project [2014BA102B01]; CAS Key Technology Talent Program; G. Harold and Leila Y. Mathers Charitable Foundation; Moxie Foundation; SCI(E); ARTICLE; 2; 243-257; 169
语种	英语
出处	SCI
出版者	CELL
内容类型	其他
源URL	[http://hdl.handle.net/20.500.11897/474075]
专题	数学科学学院 生命科学学院
推荐引用方式 GB/T 7714	Yang, Yang,Liu, Bei,Xu, Jun,et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. 2017-01-01.

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