Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L.

	Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L.
	Xu, G ; Li, CY ; Yao, YN
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2009
卷号	27 期号:2 页码:153_161
关键词	Drought Two-dimensional gel electrophoresis Hippophae rhamnoides L. Mass spectrometry
ISSN号	0735-9640
通讯作者	Yao, YN, Guizhou Univ, Coll Agr, Guiyang 550025, Peoples R China.
中文摘要	Hippophae rhamnoides L. is uniquely capable of growing well under extreme environmental conditions such as water deficit, low temperature, and high altitude. Such tolerance invokes much interest in understanding the biology of this plant species and its utilization potential. In this study, analysis of drought stress-responsive proteins in H. rhamnoides was conducted wherein greenhouse-grown seedlings were subjected to drought stress. By using proteomic techniques, proteins, extracted from leaves, were analyzed using two-dimensional electrophoresis and MALDI-TOF MS. Altogether, 55 proteins exhibited changes in abundance under stress. Of these, 13 proteins were identified, including three that disappeared under drought (a putative ABC transporter ATP-binging protein, a heat shock protein Hs1U, and a hypothetical protein XP515578), seven that were up-regulated (three large subunits of rubisco, a hypothetical protein DSM3645-23351, a putative acyl-CoA dehydrogenase, a nesprin-2, and a J-type co-chaperone HSC20), and three that were only detected under drought (a probable nitrogen regulation protein (NtrX), a 4-hydroxyphenylpyruvate dioxygenase, and an unnamed protein product). These proteins may function in beta-oxidation pathways in mitochondria, across membranes transport, abnormal protein removal, or prevent protein aggregation arrest, cell division, cytoskeleton stabilization, iron-sulfur cluster assembly, nitrogen metabolism regulation, and antioxidant substance biosynthesis. Four proteins (J-type co-chaperone Hsc20, a putative ABC transporter ATP-binging protein, NtrX, and Hs1U) were deemed as new discoveries in higher plants, and their functions were predicted either from their conserved domains or homologies to other organisms. These results provide new insights into our understanding of the mechanism of drought tolerance in plants.
学科主题	Biochemical Research Methods; Plant Sciences
收录类别	SCI
资助信息	National Natural Science Foundation of China [30525036]; China National Key Program of the International Cooperation for Science and Technology [2005DFA30620]; Science Foundation for Talents of Guizhou University [701242301]
语种	英语
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/22358]
专题	成都生物研究所_生态研究
推荐引用方式 GB/T 7714	Xu, G,Li, CY,Yao, YN. Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L.[J]. PLANT MOLECULAR BIOLOGY REPORTER,2009,27(2):153_161.
APA	Xu, G,Li, CY,&Yao, YN.(2009).Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L..PLANT MOLECULAR BIOLOGY REPORTER,27(2),153_161.
MLA	Xu, G,et al."Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L.".PLANT MOLECULAR BIOLOGY REPORTER 27.2(2009):153_161.