Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody

doi:10.1016/j.str.2018.08.012

	Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody
	Zhu, X.; Wilson, I. A.; Koudstaal, W.; Apetri, A.; Goudsmit, J.; Stoop, E. J. M.; Inganas, H.; Siregar, B.; Hoozemans, J. J.; Keogh, E.
刊名	STRUCTURE
	2018
卷号	26 期号:12 页码:1626-1634
ISSN号	0969-2126
DOI	10.1016/j.str.2018.08.012
文献子类	Article
英文摘要	Aggregation of the hyperphosphorylated protein tau into neurofibrillary tangles and neuropil threads is a hallmark of Alzheimer disease (AD). Identification and characterization of the epitopes recognized by anti-tau antibodies might shed light on the molecular mechanisms of AD pathogenesis. Here we report on the biochemical and structural characterization of a tau-specific monoclonal antibody CBTAU-24.1, which was isolated from the human memory B cell repertoire. Immunohistochemical staining with CBTAU-24.1 specifically detects pathological tau structures in AD brain samples. The crystal structure of CBTAU-24.1 Fab with a phosphorylated tau peptide revealed recognition of a unique epitope (Ser235-Leu243) in the tau proline-rich domain. Interestingly, the antibody can bind tau regardless of phosphorylation state of its epitope region and also recognizes both monomeric and paired helical filament tau irrespective of phosphorylation status. This human anti-tau antibody and its unique epitope may aid in development of diagnostics and/or therapeutic AD strategies.
电子版国际标准刊号	1878-4186
WOS关键词	PAIRED HELICAL FILAMENTS ; PHOSPHORYLATED TAU ; ALZHEIMERS-DISEASE ; PROTEIN ; PATHOLOGY ; STATE
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
WOS记录号	WOS:000454803300009
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286861]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Zhu, X.,Wilson, I. A.,Koudstaal, W.,et al. Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody[J]. STRUCTURE,2018,26(12):1626-1634.
APA	Zhu, X..,Wilson, I. A..,Koudstaal, W..,Apetri, A..,Goudsmit, J..,...&张衡.(2018).Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody.STRUCTURE,26(12),1626-1634.
MLA	Zhu, X.,et al."Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody".STRUCTURE 26.12(2018):1626-1634.