Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer | |
Yang, Dayong; Cheng LX(程林秀); Cheng, Linxiu; Zhang, Zhikun; Zuo, Duo; Zhu, Wenfeng; Zhang, Jie; Zeng, Qingdao; Li, Min; Zhao, Yuliang | |
刊名 | ACS APPLIED MATERIALS & INTERFACES |
2018 | |
卷号 | 10期号:41页码:34869-34877 |
关键词 | frequency-shift biosensing SERS ultrasensitive detection branched DNA liver cancer biomarker |
ISSN号 | 1944-8244 |
DOI | 10.1021/acsami.8b10252 |
文献子类 | Article |
英文摘要 | We provided an ultrasensitive sensing strategy for microRNA detection by first employing branched DNA. With the aid of microcontact printing, we realized the multiplex sensing of different kinds of liver cancer biomarkers: microRNA and protein simultaneously. Delicately designed branched DNA included multiple complementary sticky ends as probe to microRNA capture and the double-stranded rigid branched core to increase the active sticky-ends distance and expose more DNA probes for sensitivity. The branched DNA enables 2 orders of magnitude increase in sensitivity for microRNA detection over single-stranded DNA. The limit of detection reaches as low as 10 attomolar (S/N = 3) for miR-223 and 10(-12) M for alpha-fetoprotein. In addition, this system shows high selectivity and appropriate reproducibility (the relative standard deviation is less than 20%) in physiological media. Serum samples are tested and the results of alpha-fetoprotein are in good agreement with the current gold-standard method, electrochemiluminescence immunoassay analyzer. The results suggest the reliability of this approach in physiological media and show high potential in the sensing of low abundant microRNA in serum, especially for early diagnosis of primary liver cancers. |
WOS关键词 | ENHANCED RAMAN-SCATTERING ; HEPATOCELLULAR-CARCINOMA ; NANOPARTICLE PROBES ; STRANDED-DNA ; IMMUNOASSAY ; BIOMARKERS ; CELLS ; NANOSTRUCTURES ; SENSOR ; QUANTIFICATION |
WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
语种 | 英语 |
WOS记录号 | WOS:000447954600008 |
内容类型 | 期刊论文 |
源URL | [http://ir.ihep.ac.cn/handle/311005/286618] |
专题 | 高能物理研究所_多学科研究中心 |
作者单位 | 中国科学院高能物理研究所 |
推荐引用方式 GB/T 7714 | Yang, Dayong,Cheng LX,Cheng, Linxiu,et al. Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(41):34869-34877. |
APA | Yang, Dayong.,程林秀.,Cheng, Linxiu.,Zhang, Zhikun.,Zuo, Duo.,...&赵宇亮.(2018).Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer.ACS APPLIED MATERIALS & INTERFACES,10(41),34869-34877. |
MLA | Yang, Dayong,et al."Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer".ACS APPLIED MATERIALS & INTERFACES 10.41(2018):34869-34877. |
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