Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability

doi:10.1021/acs.jpclett.8b01570

	Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability
	Zhao, LN; Li, C; Zou CL(邹常玲); Zhao LN(赵丽娜); Feng, XD; Wang, XY; Han, BJ; Zou, CL; Hon, YH
刊名	JOURNAL OF PHYSICAL CHEMISTRY LETTERS
	2018
卷号	9 期号:16 页码:4638-4645
ISSN号	1948-7185
DOI	10.1021/acs.jpclett.8b01570
文献子类	Article
英文摘要	N-glycosylation has critical roles in regulating protein stability, but the molecular basis is poorly understood. In this study, we integrated experimental and computational techniques to investigate the mechanism by which full-length N-glycans modulate protein stability from quaternary structure perspective. We found the two inherent N-glycans of beta-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure. We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans. Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability.
WOS关键词	ENHANCED AROMATIC SEQUONS ; N-GLYCOSYLATION ; THERMOSTABILITY ; STABILIZATION ; CARBOHYDRATE ; SPECIFICITY ; CELLULOSE ; ENZYME
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
WOS记录号	WOS:000442446900026
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286241]
专题	高能物理研究所_多学科研究中心
通讯作者	Zhao LN(赵丽娜)
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Zhao, LN,Li, C,Zou CL,et al. Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2018,9(16):4638-4645.
APA	Zhao, LN.,Li, C.,邹常玲.,赵丽娜.,Feng, XD.,...&Hon, YH.(2018).Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,9(16),4638-4645.
MLA	Zhao, LN,et al."Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 9.16(2018):4638-4645.