Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics

doi:10.1186/s12951-018-0380-z

	Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics
	Xing, GM; Li, S; Ma SH(马思汉); Li J(李娟); Xing GM(邢更妹); Qin, YX; Chen, K; Gu, WH; Dong, XH; Lei, RH
刊名	JOURNAL OF NANOBIOTECHNOLOGY
	2018
卷号	16 页码:54
关键词	Fullerenol nanoparticles Metastasis Actin dynamics Young's modulus Filopodia
ISSN号	1477-3155
DOI	10.1186/s12951-018-0380-z
文献子类	Article
英文摘要	Background: Tumor metastasis is the primary cause of mortality in cancer patients. Migratory breast cancer cells in lymphatic and blood vessels seek new sites and form metastatic colonies in the lung and bone, and then these cancer cells often wreak considerable havoc. With advances in nanotechnology, nanomaterials and nanotechnologies are widely applied in tumor therapy. In this paper, small size fullerenol nanoparticles, which are separated by isoelectric focusing electrophoresis (IFE) for discrepancy of isoelectric point (pl), are used in the study of tumor metastasis. Results: In this study, the commendable inhibition of tumor metastasis was uncovered by intravenous injection of purified fullerenol fraction with special surface charge and functional groups, which was separated by IFE for discrepancy of pl. By investigating the actin dynamics in several cancer cell lines, we found these small size fullerenol nanoparticles disturbed actin dynamics. Young's modulus detection and cell migration assays revealed that fullerenol lowered stiffness and restrained migration of breast cancer cells. Filopodia, the main supporting structures of actin bundles, are important for cell motility and adhesion. Scanning electron microscopy showed that fullerenol reduced the number and length of filopodia. Simultaneously, the inhibition of integrin to form clusters on filopodias, which was likely induced by reorganizing of actin cytoskeleton, impacted cancer cell adhesion and motility. Conclusions: With intravenous injection of these fullerenol nanoparticles, tumor metastasis is well inhibited in vivo. The underlying mechanism most likely to be attributed to the effect of fullerenol nanoparticles on disturbing actin dynamics. With the disordered actin fiber, cell function is varied, including decreased cell stiffness, reduced filopodia formation, and inactivated integrin.
WOS关键词	MECHANICAL-PROPERTIES ; MATRIX DEGRADATION ; TUMOR-METASTASIS ; IN-VITRO ; CYTOSKELETON ; MIGRATION ; INVASION ; INTEGRIN ; ORGANIZATION ; REORGANIZATION
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000436142400001
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/286041]
专题	高能物理研究所_多学科研究中心高能物理研究所_加速器中心
通讯作者	Xing GM(邢更妹)
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Xing, GM,Li, S,Ma SH,et al. Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics[J]. JOURNAL OF NANOBIOTECHNOLOGY,2018,16:54.
APA	Xing, GM.,Li, S.,马思汉.,李娟.,邢更妹.,...&秦艳霞.(2018).Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics.JOURNAL OF NANOBIOTECHNOLOGY,16,54.
MLA	Xing, GM,et al."Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics".JOURNAL OF NANOBIOTECHNOLOGY 16(2018):54.