Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry

	Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry
	Hu, YM ; Wang, XM ; Fei, D ; Dong, FQ ; Ding, LS
刊名	ACTA CHIMICA SINICA
	2008
卷号	66 期号:10 页码:1245_1251
关键词	Riboflavin acridine orange herring sperm DNA action mode
ISSN号	0567-7351
其他题名	荧光光谱法研究核黄素与鲱鱼精DNA的相互作用
通讯作者	Hu, YM, SW Univ Sci & Technol, Dept Chem, Mianyang 621010, Peoples R China.
产权排序	2
中文摘要	The interaction of Riboflavin with DNA was studied in a Britton-Robinson buffer solution by employing acridine orange as a fluorescence probe. The fluorescence spectra indicated that a kind of compound of Riboflavin and DNA was formed when pH was 7.25. The binary compound ratio is n(RF) : n(DNA)= 5 : 1; the binding constant of Riboflavin with DNA compound is 2.53 x 10(6) L/mol at a temperature of 28 degrees C; and the thermodynamic parameters of the interaction by a double reciprocal method were calculated: Delta H-r(m)circle minus= -6.06 x 10(4) J/mol, Delta S-r(m)circle minus= -78.70 J/(mol.K), Delta(r)G(m)(circle minus)= -3.69x10(4) J/mol at 28 degrees C. The interaction of Riboflavin with DNA was studied also through methods of viscosity and Scatchard as well as salt effect. The results confirm that the groove binding and electrostatic binding are the two major modes of the interaction between Riboflavin and herring sperm DNA.
学科主题	Chemistry, Multidisciplinary
收录类别	SCI
语种	中文
WOS记录号	WOS:000256549500017
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/18131]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Hu, YM,Wang, XM,Fei, D,et al. Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry[J]. ACTA CHIMICA SINICA,2008,66(10):1245_1251.
APA	Hu, YM,Wang, XM,Fei, D,Dong, FQ,&Ding, LS.(2008).Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry.ACTA CHIMICA SINICA,66(10),1245_1251.
MLA	Hu, YM,et al."Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry".ACTA CHIMICA SINICA 66.10(2008):1245_1251.