Spectroscopy studies of interaction between methylene blue and herring sperm DNA

	Spectroscopy studies of interaction between methylene blue and herring sperm DNA
	Fei, D ; Wang, XM ; Li, HB ; Ding, LS ; Hu, YM ; Zhang, H ; Zhao, SL
刊名	ACTA CHIMICA SINICA
	2008
卷号	66 期号:4 页码:443_448
关键词	methylene blue acridine orange herring sperm DNA action mode
ISSN号	0567-7351
其他题名	亚甲基蓝与鲱鱼精DNA相互作用的光谱法研究
通讯作者	Fei, D, SW Univ Sci & Technol, Sch Mat Sci & Engn, Dept Chem, Mianyang 621010, Peoples R China.
产权排序	2
中文摘要	The UV-VIS spectroscopy and fluorescence spectroscopy were applied to investigate the mechanism of the interaction between methylene blue (MB) and herring sperm DNA by using acridine orange as a spectral probe in Tris-HCl buffer (pH 7.0). The chief action mode was comfirmed to be an intercalation binding at a lower MB concentration but the mixed action modes at a higher concentration. Combining ratio of the binary complex is n(MB) : n(DNA)= 10 : 1, the binding constant of MB with DNA is K-26 degrees C(circle minus)=2.46 X 10(5) L center dot mol(-1) and the apparent molor absorptivity is epsilon = 5.70 X 10(6) L center dot mol(-1)center dot cm(-1). Meanwhile, the interaction of MB with DNA was effected by the changes of pH and temperature. The results deduce that the interaction between MB and DNA is an enthalpy driven reaction.
学科主题	Chemistry, Multidisciplinary
收录类别	SCI
语种	中文
WOS记录号	WOS:000254396700008
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/18129]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Fei, D,Wang, XM,Li, HB,et al. Spectroscopy studies of interaction between methylene blue and herring sperm DNA[J]. ACTA CHIMICA SINICA,2008,66(4):443_448.
APA	Fei, D.,Wang, XM.,Li, HB.,Ding, LS.,Hu, YM.,...&Zhao, SL.(2008).Spectroscopy studies of interaction between methylene blue and herring sperm DNA.ACTA CHIMICA SINICA,66(4),443_448.
MLA	Fei, D,et al."Spectroscopy studies of interaction between methylene blue and herring sperm DNA".ACTA CHIMICA SINICA 66.4(2008):443_448.