Quantitative determination of the chemical profile of the plant material "Qiang-huo" by LC-ESI-MS-MS

	Quantitative determination of the chemical profile of the plant material "Qiang-huo" by LC-ESI-MS-MS
	Li, YH ; Jiang, SY ; Guan, YL ; Liu, X ; Zhou, Y ; Li, LM ; Huang, SX ; Sun, HD ; Peng, SL ; Zhou, Y
刊名	CHROMATOGRAPHIA
	2006
卷号	64 期号:7_8 页码:405_411
关键词	column liquid chromatography-electrospray ionization-MS-MS chromatographic fingerprints Qiang-huo Notopterygium incisum Notopterygium forbesi
ISSN号	0009-5893
通讯作者	Zhou, Y, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China.
产权排序	1
中文摘要	The rhizomes of Notopterygium incisum Ting. ex H. T. Chang and N. forbesii Boiss. are recorded in the pharmacopoeia of the People's Republic of China under the same name "Qiang-huo". Valid quality control of Qiang-huo is desirable due to the fact that the wild natural sources of Qiang-huo have almost been exhausted and large regulated cultivation is developing. in the present paper, HPLC fingerprinting was developed to identify and distinguish both species in detail. The unique properties of the HPLC fingerprint were validated by analyzing 15 batches of N. incisum and 11 batches of N. forbesii. A standardized procedure involving liquid chromatography-electrospray ionization-multiple stage mass spectrometry (HPLC-ESI-MSn) was developed to identify the fingerprint components, and a total of eight characteristic peaks were unequivocally identified. An average chromatogram from 15 batches of N. incisum from different geographic sources, considered to be the original and genuine herbal medicine, was first established as the standard fingerprint. The chemical profiles of 11 batches of N. forbesii samples were found to be variable. The HPLC method can differentiate N. incisum from N. forbesii by either the marker compounds notoptol (4) and p-hydroxypenethylanisate (8) or the amounts of nodakenin (1), notopterol (5), and 6'-O-trons-feruloylnodakenin (7). The HPLC fingerprint analysis is specific and may serve for quality identification and comprehensive evaluation of Qiang-huo.
学科主题	Biochemical Research Methods; Chemistry, Analytical
收录类别	SCI
语种	英语
WOS记录号	WOS:000241507600005
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/17827]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Li, YH,Jiang, SY,Guan, YL,et al. Quantitative determination of the chemical profile of the plant material "Qiang-huo" by LC-ESI-MS-MS[J]. CHROMATOGRAPHIA,2006,64(7_8):405_411.
APA	Li, YH.,Jiang, SY.,Guan, YL.,Liu, X.,Zhou, Y.,...&Zhou, Y.(2006).Quantitative determination of the chemical profile of the plant material "Qiang-huo" by LC-ESI-MS-MS.CHROMATOGRAPHIA,64(7_8),405_411.
MLA	Li, YH,et al."Quantitative determination of the chemical profile of the plant material "Qiang-huo" by LC-ESI-MS-MS".CHROMATOGRAPHIA 64.7_8(2006):405_411.