Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus
	Yuan, LH; Xia, JJ; Zhang, LP; Hu, CQ; cqhu@scsio.ac.cn
刊名	CONSERVATION GENETICS RESOURCES
	2012
卷号	4 期号:2 页码:303-305
关键词	Genetic Structure Microsatellite Loci Stichopus Horrens
ISSN号	1877-7252
英文摘要	This study employed the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the tropical sea cucumber species Stichopus horrens. Sixteen novel microsatellite loci were identified and we assessed the polymorphism of 40 individual samples from Hainan Province, China. The numbers of alleles ranged from 8 to 18 (total 213, mean 13.3), the observed and expected heterozygosity varied from 0.294 to 0.971, and from 0.720 to 0.913, and 12 of the 16 loci were found to be in accordance with the Hardy-Weinberg equilibrium. In addition, the successful cross-amplification of 8 loci with the congeneric Stichopus monotuberculatus, suggests that these markers could also be used in this species. These polymorphic markers can be useful for additional studies of population structure and stock management of S. horrens and related species.
学科主题	Biodiversity Conservation ; Genetics & Heredity
资助项目	LMB
语种	英语
WOS记录号	WOS:000303536400022
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9894]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	cqhu@scsio.ac.cn
推荐引用方式 GB/T 7714	Yuan, LH,Xia, JJ,Zhang, LP,et al. Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus[J]. CONSERVATION GENETICS RESOURCES,2012,4(2):303-305.
APA	Yuan, LH,Xia, JJ,Zhang, LP,Hu, CQ,&cqhu@scsio.ac.cn.(2012).Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus.CONSERVATION GENETICS RESOURCES,4(2),303-305.
MLA	Yuan, LH,et al."Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus".CONSERVATION GENETICS RESOURCES 4.2(2012):303-305.