Identification of 11 sea cucumber species by species-specific PCR method

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	Identification of 11 sea cucumber species by species-specific PCR method
	Zhang, LP; jw82123@yahoo.com.cn; Wen, J; Hu, CQ; Fan, SG
刊名	FOOD CONTROL
	2012
卷号	27 期号:2 页码:380-384
关键词	Sea Cucumber Species-specific Pcr Identification 16s Rrna Gene
ISSN号	0956-7135
英文摘要	A species-specific PCR (polymerase chain reaction) method was developed to identify the 11 sea cucumber species, even in highly processed food samples such as frozen or dried sea cucumbers (beche-de-mer). Universal primers were used for the amplification of 16S rRNA gene in each species. The alignment of the obtained sequences was the basis for the design of 11 species-specific reverse primers located in the 16S rRNA gene region. The developed species-specific PCR method was successfully applied to authenticate species of commercial sea cucumber products. Therefore, this method really provides a simple, fast and reliable protocol for the accurate identification of sea cucumber species, and also can be very useful for correct labeling of commercial products, traceability, and protection of the consumer's rights. (c) 2012 Elsevier Ltd. All rights reserved.
学科主题	Food Science & Technology
资助项目	LMB
语种	英语
WOS记录号	WOS:000305878100017
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/9798]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	jw82123@yahoo.com.cn
推荐引用方式 GB/T 7714	Zhang, LP,jw82123@yahoo.com.cn,Wen, J,et al. Identification of 11 sea cucumber species by species-specific PCR method[J]. FOOD CONTROL,2012,27(2):380-384.
APA	Zhang, LP,jw82123@yahoo.com.cn,Wen, J,Hu, CQ,&Fan, SG.(2012).Identification of 11 sea cucumber species by species-specific PCR method.FOOD CONTROL,27(2),380-384.
MLA	Zhang, LP,et al."Identification of 11 sea cucumber species by species-specific PCR method".FOOD CONTROL 27.2(2012):380-384.