In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas

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	In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas
	Yu, ZN; He, XC; Zhang, Y; carlzyu@scsio.ac.cn
刊名	MOLECULAR BIOLOGY REPORTS
	2010
卷号	37 期号:7 页码:3259-3264
关键词	Crassostrea Gigas In Silico clonIng Rieske Apoprotein Gene Expression Patterns Characterization Subcellular Localization
ISSN号	0301-4851
英文摘要	Rieske protein gene in the Pacific oyster Crassostrea gigas was obtained by in silico cloning for the first time, and its expression profiles and subcellular localization were determined, respectively. The full-length cDNA of Cgisp is 985 bp in length and contains a 5'- and 3'-untranslated regions of 35 and 161 bp, respectively, with an open reading frame of 786 bp encoding a protein of 262 amino acids. The predicted molecular weight of 30 kDa of Cgisp protein was verified by prokaryotic expression. Conserved Rieske [2Fe-2S] cluster binding sites and highly matched-pair tertiary structure with 3CWB_E (Gallus gallus) were revealed by homologous analysis and molecular modeling. Eleven putative SNP sites and two conserved hexapeptide sequences, box I (THLGC) and II (PCHGS), were detected by multiple alignments. Real-time PCR analysis showed that Cgisp is expressed in a wide range of tissues, with adductor muscle exhibiting the top expression level, suggesting its biological function of energy transduction. The GFP tagging Cgisp indicated a mitochondrial localization, further confirming its physiological function.
学科主题	Biochemistry & Molecular Biology
URL标识	查看原文
资助项目	LMB
WOS记录号	WOS:000281985400028
公开日期	2011-07-02
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/3770]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
通讯作者	carlzyu@scsio.ac.cn
推荐引用方式 GB/T 7714	Yu, ZN,He, XC,Zhang, Y,et al. In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas[J]. MOLECULAR BIOLOGY REPORTS,2010,37(7):3259-3264.
APA	Yu, ZN,He, XC,Zhang, Y,&carlzyu@scsio.ac.cn.(2010).In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas.MOLECULAR BIOLOGY REPORTS,37(7),3259-3264.
MLA	Yu, ZN,et al."In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas".MOLECULAR BIOLOGY REPORTS 37.7(2010):3259-3264.