Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus

	Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus
	Jia, YH ; Li, DS ; Zhu, SW ; Zhang, LY ; Ding, LS ; Wang, WY ; Xiong, YL
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2003
卷号	35 期号:7 页码:619_623
关键词	angiotensin-converting enzyme bradykinin bradykinin-potentiating peptide TmF
ISSN号	0582-9879
通讯作者	Xiong, YL, Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxicol, Kunming 650223, Peoples R China.
产权排序	3
中文摘要	A novel bradykinin-potentiating peptide (BPP), designated as TmF, has been purified to homogeneity from the venom of Trimeresurus mucrosquamatus by 70% cold methanol extraction, Sephadex G-15 gel filtration and reverse-phase high performance liquid chromatography (RP-HPLC). The amino acid sequence of TmF was determined to be pGlu-Gly-Arg-Pro-Leu-Gly-Pro-Pro-Ile-Pro-Pro (pGlu denotes pyroglutamic acid), which shared high homology with other BPPs. The molecular mass of TmF was 1.1107 kD as determinated by electrospray ionization-mass spectrometry (ESI-MS), which was in accordance with the calculated value of 1.1106 kD. The potentiating "unit" of TmF to bradykinin-induced (BK-induced) contraction on the guinea-pig ileum in vitro was (1.13 +/- 0.3) unit (mg/L), and TmF (5.0 x 10(-4) mg/kg) increased the pressure-lowering-effect of bradykinin (5.0 x 10(-5) mg/kg) with approximate descent value of (14 +/- 2) mmHg. In addition, TmF inhibited the conversion of angiotensin I to angiotensin 11, 2 x 10(-3) mg of TmF caused 50% inhibition (IC50) of angiotensin-converting enzyme (ACE) hydrolyzing activity to bradykinin.
学科主题	Biochemistry & Molecular Biology; Biophysics
收录类别	SCI
语种	英语
WOS记录号	WOS:000184466500005
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/17385]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Jia, YH,Li, DS,Zhu, SW,et al. Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(7):619_623.
APA	Jia, YH.,Li, DS.,Zhu, SW.,Zhang, LY.,Ding, LS.,...&Xiong, YL.(2003).Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(7),619_623.
MLA	Jia, YH,et al."Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.7(2003):619_623.