Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives

doi:10.1016/j.fsi.2018.10.058

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives
	Zhou, Sheng ; Qin, Qiwei ; Xiao, Su-Mei ; Wang, Wenxiong 1; Huang, Youhua ; Wei, Jingguang ; Wang, Sunli ; Zhou, Xuefeng 4; Mao, Xinliang ; Zhou, Xiaofeng
刊名	FISH & SHELLFISH IMMUNOLOGY
	2019
卷号	84 页码:1115
关键词	Bacillus subtilis Probiotic Grouper Growth Innate immunity
ISSN号	1050-4648
DOI	10.1016/j.fsi.2018.10.058
英文摘要	Probiotics are widely used for the improvement of animals' growth and health. However, few marine aquatic probiotics are applied and licensed in China. In this study, a Bacillus spp. strain was isolated from the Hulong grouper gastrointestinal tract, which was identified as a new strain of Bacillus subtilis and was named as 7k. B. subtilis 7k showed desirable capability of sporulation and resistance to heat, simulated gastric juice and simulated duodenum juice, indicating its potential as probiotics. Seven antimicrobial chemicals were found in the secretion of the B. subtilis 7k. B. subtilis 7k addition in diet promoted the growth rate of Hulong groupers. Moreover, B. subtilis 7k can inhibit infection by iridovirus, making B. subtilis 7k a suitable kind of probiotic for maintaining fishes' health. Our results also revealed that B. subtilis 7k induced non-specific immune response in Hulong grouper under virus infection. Hulong grouper fed by diets containing B. subtilis 7k at 10(8) and 10(10) cfu g(-1) for 4-8 weeks were significantly strengthened in serum lysozyme activity, serum alternative complement activity (ACH50), serum bactericidal activity, respiratory burst, superoxide dismutase activity (SOD), and phagocytic activity of head kidney leucocytes when compared with those fed by control diets. In conclusion, B. subtilis 7k was isolated and characterized to be a kind of process enduring, growth stimulating, immunity enhancing and health promoting probiotic using in grouper culture.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17924]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.Chinese Acad Sci, South China Sea Inst Oceanol, Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 2.South China Agr Univ, Coll Marine Sci, Joint Lab Guangdong Prov & Hong Kong Reg Marine B, Guangzhou 510642, Guangdong, Peoples R China 3.Sun Yat Sen Univ, Sch Publ Hlth, Dept Med Stat & Epidemiol, Guangzhou 510080, Guangdong, Peoples R China 4.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Pharmacol, Guangzhou 510080, Guangdong, Peoples R China 5.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Kowloon, Clearwater Bay, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Sheng,Qin, Qiwei,Xiao, Su-Mei,et al. Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:1115, 1124.
APA	Zhou, Sheng.,Qin, Qiwei.,Xiao, Su-Mei.,Wang, Wenxiong.,Huang, Youhua.,...&Song, Deli.(2019).Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives.FISH & SHELLFISH IMMUNOLOGY,84,1115.
MLA	Zhou, Sheng,et al."Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E-lanceolatus) and its effects as probiotic additives".FISH & SHELLFISH IMMUNOLOGY 84(2019):1115.