Characterization of a new microbial Pictet-Spenglerase NscbB affording the beta-carboline skeletons from Nocardiopsis synnemataformans DSM 44143 | |
Chen, Qi; Xie, Yunchang1; Zhang, Shaofei2 | |
刊名 | JOURNAL OF BIOTECHNOLOGY
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2018 | |
卷号 | 281页码:137 |
关键词 | Pictet-Spengler reaction Pictet-Spenglerase beta-carboline alkaloid NscbB McbB Nocardiopsis synnemataformans |
ISSN号 | 0168-1656 |
英文摘要 | The enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of beta-carboline (beta C) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic beta-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplant patient derived) by homologous alignment with its well-characterized counterpart McbB. The biochemical analysis showed that NscbB could catalyze L-tryptophan (K-M = 89.64 +/- 8.69 mu M) and methylglyoxal (K-M = 147.70 +/- 16.38 mu M), without cofactors, to form the two beta C skeletons 1-acetyl-3-carboxy-beta-carboline and 1-acetyl-beta-carboline in vitro. Additionally, the heterologous expression of nscbB in E. coli BL21 (DE3) revealed the efficient bioproductivity of NscbB to bioproduce two beta C skeletons, 1-acetyl-3-carboxy-beta-carboline (5.5 mg/L) and 1-acetyl-beta-carboline (3.1 mg/L), within 16 h fermentation. These results demonstrate NscbB is a novel and practical microbial PSase, which is useful for future bioactive beta C alkaloid exploitation and development. |
内容类型 | 期刊论文 |
源URL | [http://ir.scsio.ac.cn/handle/344004/17635] ![]() |
专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
作者单位 | 1.Anhui Med Univ, Sch Pharm, Hefei 230032, Anhui, Peoples R China 2.Anhui Med Univ, Sch Life Sci, Hefei 230032, Anhui, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Qi,Xie, Yunchang,Zhang, Shaofei. Characterization of a new microbial Pictet-Spenglerase NscbB affording the beta-carboline skeletons from Nocardiopsis synnemataformans DSM 44143[J]. JOURNAL OF BIOTECHNOLOGY,2018,281:137, 143. |
APA | Chen, Qi,Xie, Yunchang,&Zhang, Shaofei.(2018).Characterization of a new microbial Pictet-Spenglerase NscbB affording the beta-carboline skeletons from Nocardiopsis synnemataformans DSM 44143.JOURNAL OF BIOTECHNOLOGY,281,137. |
MLA | Chen, Qi,et al."Characterization of a new microbial Pictet-Spenglerase NscbB affording the beta-carboline skeletons from Nocardiopsis synnemataformans DSM 44143".JOURNAL OF BIOTECHNOLOGY 281(2018):137. |
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