| On-line capillary electrophoresis mass spectrometry for determination of peptide mixture and protein mixture | |
| Zhou, GH; Luo, GA; Zhou, KY; Gu, ZL | |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 1998-03-01 | |
| 卷号 | 19期号:3页码:353-358 |
| 关键词 | capillary electrophoresis-mass spectrometry coated capillary peptide protein tryptic mapping of erythropoietin |
| ISSN号 | 0251-0790 |
| 通讯作者 | Luo, GA() |
| 英文摘要 | The influence of uncoated, coated linear polyacrylamide(LPA) and amino-coated capillaries on the separation of five peptide mixtures, including angiotensin I, II, III, substance P and somatostain, was studied. The results showed that the peptide mixtures could not be separated completely in uncoated capillary by volatile buffer which was suitable for the MS analysis, whereas they could be separated in LPA and amino coated capillary by pH 5.0 and pH 4.5 NH4Ac buffer respectively. In addition, the above peptide mixtures, protein mixtures comprised of myoglobin and carbonic anhydrase II, tryptic digests of erythropoietin, were separated and characterized by capillary electrophoresis-mass spectrometry(CE-ESI-MS) with electrospray ionization interface. The factors affecting the determination in CE-ESI-MS were also discussed. |
| 收录类别 | SCI |
| WOS关键词 | ZONE ELECTROPHORESIS |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | HIGHER EDUCATION PRESS |
| WOS记录号 | WOS:000072677800006 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2559100 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Luo, GA |
| 作者单位 | 1.Tsing Hua Univ, Sch Life Sci & Engn, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Inst Soil Sci, Nanjing 210018, Peoples R China 3.Nanjing Mil Inst Drug Control, Nanjing 210002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, GH,Luo, GA,Zhou, KY,et al. On-line capillary electrophoresis mass spectrometry for determination of peptide mixture and protein mixture[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,1998,19(3):353-358. |
| APA | Zhou, GH,Luo, GA,Zhou, KY,&Gu, ZL.(1998).On-line capillary electrophoresis mass spectrometry for determination of peptide mixture and protein mixture.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,19(3),353-358. |
| MLA | Zhou, GH,et al."On-line capillary electrophoresis mass spectrometry for determination of peptide mixture and protein mixture".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 19.3(1998):353-358. |
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