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	Application of capillary electrophoresis, liquid chromatography, electrospray mass spectrometry and matrix-assisted laser desorption/ionization time of flight mass spectrometry to the characterization of recombinant human erythropoietin
	Zhou, GH; Luo, GA; Zhou, Y; Zhou, KY; Zhang, XD; Huang, LQ
刊名	ELECTROPHORESIS
	1998-10-01
卷号	19期号:13页码:2348-2355
关键词	recombinant human erythropoietin high performance capillary electrophoresis high performance liquid chromatography electrospray mass spectrometry matrix-assisted laser desorption ionization time of flight mass spectrometry
ISSN号	0173-0835
通讯作者	Luo, GA()
英文摘要	High performance capillary electrophoresis (HPCE), high performance liquid chromatography (HPLC), matrix-assisted laser desorption/ionization - time of flight - mass spectrometry (MALDI-TOF-MS), on-line CE-electrospray ionization-mass spectrometry (CE-ESI-MS) and on-line LC-ESI-MS have been employed to characterize a heterogeneous glycoprotein, recombinant human erythropoietin (rHuEPO) expressed from Chinese hamster ovary (CHO) cells. The analysis was demonstrated through two specific levels of detail: the intact protein and tryptic digests of the protein. Six glycoforms of rHuEPO were separated by HPCE; seventeen tryptic fragments in a total of ZI nonglycosylated and glycosylated peptides were characterized; the O-linked glycopeptides were analyzed directly by CE-ESI-MS and LC-ESI-MS. In particular, four glycans of O-acetylation of sialic acid were identified in the O-linked glycosylated fragments. The molecular weight of rHuEPO was accurately determined by MALDI-TOF-MS.
收录类别	SCI
WOS关键词	CARBOHYDRATE STRUCTURE ; MICROHETEROGENEITY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000076280000014
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2559091
专题	南京土壤研究所
通讯作者	Luo, GA
作者单位	1.Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China 2.Nanjing Mil Area Inst Drug Control, Nanjing, Peoples R China 3.Chinese Acad Sci, Inst Soil Sci, Nanjing, Peoples R China
推荐引用方式 GB/T 7714	Zhou, GH,Luo, GA,Zhou, Y,et al. Application of capillary electrophoresis, liquid chromatography, electrospray mass spectrometry and matrix-assisted laser desorption/ionization time of flight mass spectrometry to the characterization of recombinant human erythropoietin[J]. ELECTROPHORESIS,1998,19(13):2348-2355.
APA	Zhou, GH,Luo, GA,Zhou, Y,Zhou, KY,Zhang, XD,&Huang, LQ.(1998).Application of capillary electrophoresis, liquid chromatography, electrospray mass spectrometry and matrix-assisted laser desorption/ionization time of flight mass spectrometry to the characterization of recombinant human erythropoietin.ELECTROPHORESIS,19(13),2348-2355.
MLA	Zhou, GH,et al."Application of capillary electrophoresis, liquid chromatography, electrospray mass spectrometry and matrix-assisted laser desorption/ionization time of flight mass spectrometry to the characterization of recombinant human erythropoietin".ELECTROPHORESIS 19.13(1998):2348-2355.

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