| Rapid detection of Salmonella species using an improved gel-based DNA microarray method | |
| Ye, Xuhong1,2,3,4; Wang, Yiming1,2,3; Lin, Xiangui1,2,3 | |
| 刊名 | AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
 |
| 2011-08-04 | |
| 卷号 | 5期号:15页码:2095-2099 |
| 关键词 | Salmonella gyrB gene PCR DNA microarray TEMED |
| ISSN号 | 1996-0808 |
| 通讯作者 | Lin, Xiangui(xglin@issas.ac.cn) |
| 英文摘要 | Salmonella, widely distributed in nature, is a great human and animal health hazard of a class of pathogens. Culture-based methods may require many days to detect Salmonella. Traditional microbiology could advantageously be replaced by DNA microarray technology. We described an improved 3-D polyacrylamide gel-based DNA microarray assay based on gyrB gene (DNA gyrase B subunit gene) sequences that can be used for the identification of Salmonella species. Primers specific for a gyrB gene region common to all 13 samples were synthesized and used for PCR amplification of purified DNA. An oligonucleotide probe for specific gyrB gene regions was developed for the identification of 7 Salmonella species. Acrylamide-modified oligonucleotides solutions containing acrylamide monomer, glycerol, APS and probe were prepared at the desired concentration. The solutions were spotted on the modified glass slide by ink jet using a microarrayer and then the slide was transferred to a vacuum chamber with TEMED, after that the slide was used for hybridization with fluorescently labeled ssDNA derived from amplified sample DNA to yield a pattern of positive spots. This microarray produced unique hybridization patterns for species of Salmonella and could differentiate closely related bacterial species. The sample preparation and microarray method used in this study increased sensitivity and reduces time-to-result for detection of Salmonella. The described method allowed microarray monitoring for Salmonella contamination of food and manure for aquaculture. |
| 收录类别 | SCI |
| WOS关键词 | ESCHERICHIA-COLI ; GYRB GENE ; IDENTIFICATION ; HYBRIDIZATION ; SEQUENCES |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| 出版者 | ACADEMIC JOURNALS |
| WOS记录号 | WOS:000294668400015 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2558886 |
| 专题 | 南京土壤研究所 |
| 通讯作者 | Lin, Xiangui |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Beijing 210008, Peoples R China 2.Hongkong Baptist Univ, Joint Open Lab Soil & Environm, Nanjing 210008, Peoples R China 3.Chinese Acad Sci, Inst Soil Sci, Nanjing 210008, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Xuhong,Wang, Yiming,Lin, Xiangui. Rapid detection of Salmonella species using an improved gel-based DNA microarray method[J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,2011,5(15):2095-2099. |
| APA | Ye, Xuhong,Wang, Yiming,&Lin, Xiangui.(2011).Rapid detection of Salmonella species using an improved gel-based DNA microarray method.AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,5(15),2095-2099. |
| MLA | Ye, Xuhong,et al."Rapid detection of Salmonella species using an improved gel-based DNA microarray method".AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 5.15(2011):2095-2099. |
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