QQ客服 官方微博 反馈留言

	Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus
	Liu, Lu1,2; Hu, Wei1; Li, Wen-jian1; Wang, Shu-yang1; Lu, Dong1; Tian, Xue-jiao1,2; Mao, Yan-qin1,2; Liu, Jing1; Chen, Ji-hong1
刊名	ENGINEERING IN LIFE SCIENCES
	2019-02-01
卷号	19期号:2页码:112-120
关键词	fermentation industry heavy ion mutagenesis mutation tool screening process Streptomyces fungicidicus
ISSN号	1618-0240
DOI	10.1002/elsc.201800109
通讯作者	Hu, Wei(hudongli007@impcas.ac.cn) ; Chen, Ji-hong(chjh@impcas.ac.cn)
英文摘要	To improve fermentative production of enduracidin, heavy-ion beams generated by the Heavy Ion Research Facility in Lanzhou (HIRFL), China, were employed for the first time to generate mutations in Streptomyces fungicidicus. Initial screening detected 44 positive mutants with larger inhibition zone, which were subsequently tested based on flask fermentation. Finally, 20 mutants showed 20% increase in enduracidin production, when compared with the original strain. Among them, enduracidin production by the three mutants (M13, M30, and M34) was significantly higher than that by the original strain. In particular, mutant M30 exhibited highest enduracidin production, which was 114% higher than that obtained with the original strain. Following culture optimization, the maximal enduracidin yield obtained by M30 reached 918.5 mg/L in 10 days, which was 34% higher than that noted in the control.
收录类别	SCI
WOS关键词	MUTATION ; BEAMS
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000457650400003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2558170
专题	寒区旱区环境与工程研究所
通讯作者	Hu, Wei; Chen, Ji-hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lu,Hu, Wei,Li, Wen-jian,et al. Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus[J]. ENGINEERING IN LIFE SCIENCES,2019,19(2):112-120.
APA	Liu, Lu.,Hu, Wei.,Li, Wen-jian.,Wang, Shu-yang.,Lu, Dong.,...&Chen, Ji-hong.(2019).Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus.ENGINEERING IN LIFE SCIENCES,19(2),112-120.
MLA	Liu, Lu,et al."Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus".ENGINEERING IN LIFE SCIENCES 19.2(2019):112-120.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。