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	Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana
	Zhao, Pengshan1,2; Liu, Fei2; Ma, Miao2; Gong, Jiao2; Wang, Qiujun2; Jia, Pengfei2; Zheng, Guochang2; Liu, Heng2
刊名	MOLECULAR BIOLOGY
	2011-10-01
卷号	45期号:5页码:785-796
关键词	ABA AtLEA3-3 osmotic stress salt stress seedling establishment transgenic plant
ISSN号	0026-8933
DOI	10.1134/S0026893311050165
通讯作者	Zhao, Pengshan()
英文摘要	Previous studies have shown that the late embryogenesis abundant (LEA) group 3 proteins significantly respond to changes in environmental conditions. However, reports that demonstrate their biological role, especially in Arabidopsis, are notably limited. This study examines the functional roles of the Arabidopsis LEA group 3 proteins AtLEA3-3 and AtLEA3-4 in abiotic stress and ABA treatments. Expression of AtLEA3-3 and AtLEA3-4 is upregulated by ABA, high salinity, and osmotic stress. Results on the ectopic expression of AtLEA3-3 and AtLEA3-4 in E. coli suggest that both proteins play important roles in resistance to cold stress. Overexpression of AtLEA3-3 in Arabidopsis (AtLEA3-3-OE) confers salt and osmotic stress tolerance that is characterized during germination and early seedling establishment. However, AtLEA3-3-OE lines show sensitivity to ABA treatment during early seedling development. These results suggest that accumulation of AtLEA3-3 mRNA and/or proteins may help heterologous ABA reinitiate second dormancy during seedling establishment. Analysis of yellow fluorescent fusion proteins localization shows that AtLEA3-3 and AtLEA3-4 are mainly distributed in the ER and that AtLEA3-3 also localizes in the nucleus, and in response to salt, mannitol, cold, or BFA treatments, the localization of AtLEA3-3 and AtLEA3-4 is altered and becomes more condensed. Protein translocalization may be a positive and effective strategy for responding to abiotic stresses. Taken together, these results suggest that AtLEA3-3 has an important function during seed germination and seedling development of Arabidopsis under abiotic stress conditions.
收录类别	SCI
WOS关键词	EMBRYOGENESIS ABUNDANT PROTEIN ; RESPONSIVE GENE-EXPRESSION ; TRANSCRIPTION FACTOR ; LEA PROTEINS ; WATER-DEFICIT ; FUNCTIONAL-ANALYSIS ; SALT TOLERANCE ; IN-VITRO ; DESICCATION ; GERMINATION
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	MAIK NAUKA/INTERPERIODICA/SPRINGER
WOS记录号	WOS:000295681900010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2556616
专题	寒区旱区环境与工程研究所
通讯作者	Zhao, Pengshan
作者单位	1.Chinese Acad Sci, Lab Plant Stress Ecophysiol & Biotechnol, Shapotou Desert Res & Expt Stn, Cold & Arid Regions Environm & Engn Res Inst, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, MOE Key Lab Arid & Grassland Ecol, Inst Cell Biol, Sch Life Sci, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Pengshan,Liu, Fei,Ma, Miao,et al. Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana[J]. MOLECULAR BIOLOGY,2011,45(5):785-796.
APA	Zhao, Pengshan.,Liu, Fei.,Ma, Miao.,Gong, Jiao.,Wang, Qiujun.,...&Liu, Heng.(2011).Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana.MOLECULAR BIOLOGY,45(5),785-796.
MLA	Zhao, Pengshan,et al."Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana".MOLECULAR BIOLOGY 45.5(2011):785-796.

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